About: Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.     Goto   Sponge   NotDistinct   Permalink

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  • Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.
  • Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters. (en)
Title
  • Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.
  • Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58. (en)
skos:prefLabel
  • Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.
  • Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58. (en)
skos:notation
  • RIV/00216224:14310/11:00049448!RIV12-AV0-14310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0001/01/01), P(IAA401630901), P(LC06010), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
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  • 188248
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/11:00049448
http://linked.open...riv/jazykVysledku
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  • Novel Haloalkane Dehalogenase DatA; Agrobacterium tumefaciens C58 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [94D1B633461D]
http://linked.open...i/riv/nazevZdroje
  • Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 77
http://linked.open...iv/tvurceVysledku
  • Chaloupková, Radka
  • Damborský, Jiří
  • Fořtová, Andrea
  • Hasan, Khomaini
  • Ishitsuka, M.
  • Koudeláková, Táňa
  • Nagata, Yuji
  • Prokop, Zbyněk
http://linked.open...ain/vavai/riv/wos
  • 000287700100043
http://linked.open...n/vavai/riv/zamer
issn
  • 0099-2240
number of pages
http://bibframe.org/vocab/doi
  • 10.1128/AEM.02109-10
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  • 14310
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