About: Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa

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About: Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Random mutagenesis is the most useful approach in directed evolution, widely used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries. One of these methods is error-prone PCR. This technique introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and mutational reaction conditions. LecB is the target gene we have modified via random mutagenesis. This gene codes PA-IIL lectin from Pseudomonas aeruginosa. This lectin is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present in cystic fibrosis mucins. The random mutagenesis could help us to create a protein with interestingly modified properties towards monospecificity. Random mutagenesis is the most useful approach in directed evolution, widely used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries. One of these methods is error-prone PCR. This technique introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and mutational reaction conditions. LecB is the target gene we have modified via random mutagenesis. This gene codes PA-IIL lectin from Pseudomonas aeruginosa. This lectin is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present in cystic fibrosis mucins. The random mutagenesis could help us to create a protein with interestingly modified properties towards monospecificity. (en)
Title	Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa (en)
skos:prefLabel	Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa (en)
skos:notation	RIV/00216224:14310/10:00057485!RIV13-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD301/09/H004), Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Pokorná, Martina Wimmerová, Michaela Mrázková, Jana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	283874
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00057485
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	random mutagenesis; lectins; PA-IIL; Pseudomonas aeruginosa (en)
http://linked.open.../riv/klicoveSlovo	lectins Pseudomonas aeruginosa PA-IIL random mutagenesis
http://linked.open...ontrolniKodProRIV	[ECBAFE059208]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Mrázková, Jana Pokorná, Martina Wimmerová, Michaela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14310

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