About: 18O-based protein quantitation: influence of separation on quantitation outcome in analysis of Staphylococcus a. enterotoxins

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About: 18O-based protein quantitation: influence of separation on quantitation outcome in analysis of Staphylococcus a. enterotoxins Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Food contamination by enterotoxins from Staphylococcus aureus is of intense interest from side of food microbiologists and biochemists. Our work is focused on enterotoxin H (SEH). One of the main aims of our work is to reliably determine the content of SEH in whole secretome, on a background of all other staphylococcal enterotoxins that are partially sequence homologous. Mass spectrometry driven protein quantitation seems to be the most selective way. Prior to the quantitation, proteins must be separated. As the proteins are most conveniently quantified via their tryptic peptides, the separation should ensure availability of a quantifiable tryptic peptide. Here we present a study on influence of different separation ways, namely polyacrylamide gel electrophoresis, reversed phase liquid chromatography and off-gel isoelectric focusation, on availability of quantifiable tryptic peptides of SEH for 18O-based protein quantitation routine. Food contamination by enterotoxins from Staphylococcus aureus is of intense interest from side of food microbiologists and biochemists. Our work is focused on enterotoxin H (SEH). One of the main aims of our work is to reliably determine the content of SEH in whole secretome, on a background of all other staphylococcal enterotoxins that are partially sequence homologous. Mass spectrometry driven protein quantitation seems to be the most selective way. Prior to the quantitation, proteins must be separated. As the proteins are most conveniently quantified via their tryptic peptides, the separation should ensure availability of a quantifiable tryptic peptide. Here we present a study on influence of different separation ways, namely polyacrylamide gel electrophoresis, reversed phase liquid chromatography and off-gel isoelectric focusation, on availability of quantifiable tryptic peptides of SEH for 18O-based protein quantitation routine. (en)
Title	18O-based protein quantitation: influence of separation on quantitation outcome in analysis of Staphylococcus a. enterotoxins 18O-based protein quantitation: influence of separation on quantitation outcome in analysis of Staphylococcus a. enterotoxins (en)
skos:prefLabel	18O-based protein quantitation: influence of separation on quantitation outcome in analysis of Staphylococcus a. enterotoxins 18O-based protein quantitation: influence of separation on quantitation outcome in analysis of Staphylococcus a. enterotoxins (en)
skos:notation	RIV/00216224:14310/10:00049635!RIV12-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/09/0148), Z(MSM0021622413), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Župková, Marcela Zdráhal, Zbyněk Havliš, Jan Nováková, Kateřina
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	300853
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00049635
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	quantitation; mass spectrometry; staphylococcal enterotoxins; separation (en)
http://linked.open.../riv/klicoveSlovo	mass spectrometry separation staphylococcal enterotoxins quantitation
http://linked.open...ontrolniKodProRIV	[700A52BC9B91]
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Modern methods of detection and determination of microbial toxins
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Nováková, Kateřina Zdráhal, Zbyněk Havliš, Jan Župková, Marcela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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