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  • The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter Microbial Characterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2).
  • The human gastrointestinal tract contains a diverse microbial community. Among them lactobacilli are important members of the natural intestinal microbiota playing a beneficial role in balancing of the intestinal environment. A collection of 51 Lactobacillus isolates originating from children intestinal mucous tissues has been studied. Analysed biopsy samples were retrieved during a routine endoscopic examination of different parts of the large intestine in patients revealing various disorders. A combined approach, which included automated ribotyping and MALDI-TOF MS analysis, was applied for identification of isolated Lactobacillus spp. in order to compare the discriminatory power of these methods. Automated ribotyping using the RiboPrinter Microbial Characterization System (DuPont Qualicon) assigned 38 lactobacilli to the species level: Lactobacillus salivarius (29 isolates), Lactobacillus paracasei subsp. paracasei (7) and Lactobacillus rhamnosus (2). (en)
Title
  • Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
  • Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS (en)
skos:prefLabel
  • Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS
  • Identification of intestinal lactobacilli by automated ribotyping and MALDI-TOF MS (en)
skos:notation
  • RIV/00216224:14310/10:00045193!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B08068), P(LC06034), Z(MSM0021622415), Z(MSM0021622416)
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  • 262702
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  • RIV/00216224:14310/10:00045193
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  • Identification; Lactobacillus; MALDI-TOF MS; RiboPrinter (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [16021D742CD9]
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  • Dráb, Vladimír
  • Sedláček, Ivo
  • Teshim, Andrea
  • Zdráhal, Zbyněk
  • Šedo, Ondrej
  • Švec, Pavel
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14310
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