About: Methods of directed evolution of PA-IIL lectin

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Directed evolution is a powerful tool widely used in protein engineering for producing proteins with altered or improved properties. This approach includes a lot of methods used to create diverse mutant libraries and to screen these libraries. In our work two mutation techniques are being used. The first one is random mutagenesis by error-prone PCR (which introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and reaction conditions). The second one is targeted-random mutagenesis (which employs mixture of special primers carried random base substitutions on selected site). LecB, the gene coding PA-IIL lectin from bacterium Pseudomonas aeruginosa, was chosen as a target gene to be modified via random mutagenesis. This soluble protein is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present in cystic fibrosis mucins. Directed evolution is a powerful tool widely used in protein engineering for producing proteins with altered or improved properties. This approach includes a lot of methods used to create diverse mutant libraries and to screen these libraries. In our work two mutation techniques are being used. The first one is random mutagenesis by error-prone PCR (which introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and reaction conditions). The second one is targeted-random mutagenesis (which employs mixture of special primers carried random base substitutions on selected site). LecB, the gene coding PA-IIL lectin from bacterium Pseudomonas aeruginosa, was chosen as a target gene to be modified via random mutagenesis. This soluble protein is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present in cystic fibrosis mucins. (en)
Title	Methods of directed evolution of PA-IIL lectin Methods of directed evolution of PA-IIL lectin (en)
skos:prefLabel	Methods of directed evolution of PA-IIL lectin Methods of directed evolution of PA-IIL lectin (en)
skos:notation	RIV/00216224:14310/10:00044241!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA303/09/1168), P(GD301/09/H004), P(LC06030), S, Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Pokorná, Martina Wimmerová, Michaela Mrázková, Jana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	270725
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00044241
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	directed evolution; random mutagenesis in vitro; targeted-random mutagenesis; lectin PA-IIL; Pseudomonas aeruginosa (en)
http://linked.open.../riv/klicoveSlovo	Pseudomonas aeruginosa directed evolution random mutagenesis in vitro targeted-random mutagenesis lectin PA-IIL
http://linked.open...ontrolniKodProRIV	[56C9115063C9]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Lectins from human pathogens - structure, function, engineering Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors Biomolecular centre
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Mrázková, Jana Pokorná, Martina Wimmerová, Michaela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14310

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