About: Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation. The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation. (en)
Title	Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach (en)
skos:prefLabel	Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach (en)
skos:notation	RIV/00216224:14310/10:00043613!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GP203/07/P471), S, Z(MSM0021622413), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Struhárová, Iva Bouchal, Pavel Kučera, Igor Vyhlídalová, Tereza Budinská, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	294406
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00043613
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Paracoccus denitrificans; FNR transcription regulator; Respiratory switch; Two-dimensional gel electrophoresis; Proteome (en)
http://linked.open.../riv/klicoveSlovo	Paracoccus denitrificans Proteome FNR transcription regulator Respiratory switch Two-dimensional gel electrophoresis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[F1225EFE18E3]
http://linked.open...i/riv/nazevZdroje	Biochimica et Biophysica Acta - Proteins and Proteomics
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	1804
http://linked.open...iv/tvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Bouchal, Pavel Kučera, Igor Struhárová, Iva Vyhlídalová, Tereza Budinská, Eva van Spanning, Rob
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím Molecular basis of cell and tissue regulations
issn	1570-9639
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach

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