About: Ribotyping and biotyping of Lactobacillus helveticus from the koumiss.

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About: Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Lactobacillus helveticus, a homofermentative thermophilic lactobacillus commonly occurring in cheeses, has been isolated as the prevailing species in natural koumiss in this work. The species identification of six studied L. helveticus strains was based on rep-PCR fingerprinting with (GTG)5 primer. Biotyping (API 50CH kit, conventional tests) shown phenotypic heterogeneity among isolates and enabled the identification to the genus Lactobacillus only. Ribotyping with restriction enzyme EcoRI yielded a strain - specific restriction patterns and allowed good strain differentiation. Despite the low number of strains analyzed, the ribotype data showed certain heterogeneity, that seemed to be not only strain dependent, but also related to the source of isolates. We concluded that analyzed isolates from koumiss represent a new ecovar, L. helveticus ecovar Koumiss. Lactobacillus helveticus, a homofermentative thermophilic lactobacillus commonly occurring in cheeses, has been isolated as the prevailing species in natural koumiss in this work. The species identification of six studied L. helveticus strains was based on rep-PCR fingerprinting with (GTG)5 primer. Biotyping (API 50CH kit, conventional tests) shown phenotypic heterogeneity among isolates and enabled the identification to the genus Lactobacillus only. Ribotyping with restriction enzyme EcoRI yielded a strain - specific restriction patterns and allowed good strain differentiation. Despite the low number of strains analyzed, the ribotype data showed certain heterogeneity, that seemed to be not only strain dependent, but also related to the source of isolates. We concluded that analyzed isolates from koumiss represent a new ecovar, L. helveticus ecovar Koumiss. (en)
Title	Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. (en)
skos:prefLabel	Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. (en)
skos:notation	RIV/00216224:14310/10:00043390!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B08068), Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Nováková, Dana Sedláček, Ivo Švec, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	285278
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00043390
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Lactobacillus helveticus-Ribotyping-Rep-PCR-Koumiss-Biotyping-Identification (en)
http://linked.open.../riv/klicoveSlovo	Lactobacillus helveticus-Ribotyping-Rep-PCR-Koumiss-Biotyping-Identification
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[752EFCB37B08]
http://linked.open...i/riv/nazevZdroje	European Food Research Technology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Research into possibilities of application both automated and semi-automated methods for identification and describing new microorganisms with probiotic properties with aim to extend specific genetic sources for civilizing diseases prevention.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	230
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Nováková, Dana
http://linked.open...ain/vavai/riv/wos	000274385400009
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
issn	1438-2377
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. Ribotyping and biotyping of Lactobacillus helveticus from the koumiss. Ribotyping and biotyping of Lactobacillus helveticus from the koumiss.

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