About: Determination of capsidiol in tobacco cells culture by HPLC

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. Capsidiol is a bicyclic sesquiterpene, which accumulates extracellularly in plants, and has been isolated from many types of Solanaceae. It acts as a phytoalexin produced by Nicotiana tabacum in response to pathogens. Capsidiol has antifungal activity and is formed as first in tobacco and pepper plants after infestation. The amount of capsidiol in tobacco cell suspension culture has been previously determined by solid-phase extraction and organic solvent extraction with TLC or GC analysis. An HPLC method with UV detection at 210 nm on a C8 column utilizing both extraction methods was developed to analyze capsidiol in suspension cell culture. The HPLC method was linear in the concentration range of 0.1-2.0 mg/l. The lower limit of quantitation was 0.1 mg/l. Organic solvent extraction and solid phase extraction methods were compared. Both methods are generally similar in their overall efficiency (82% and 75%,respectively) but eliminations of interfering compounds are different. (en)
Title	Determination of capsidiol in tobacco cells culture by HPLC Determination of capsidiol in tobacco cells culture by HPLC (en)
skos:prefLabel	Determination of capsidiol in tobacco cells culture by HPLC Determination of capsidiol in tobacco cells culture by HPLC (en)
skos:notation	RIV/00216224:14310/10:00042276!RIV11-GA0-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA522/06/0156), P(ME 942), S, Z(MSM0021622413), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Kašparovský, Tomáš Lochman, Jan Literáková, Petra Mikeš, Vladimír Zdráhal, Zbyněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	253735
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00042276
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capsidiol Tobacco HPLC (en)
http://linked.open.../riv/klicoveSlovo	Capsidiol Tobacco HPLC
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[5A9749374825]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatographic Science
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure-function relationship of elicitors from Phytophthora spp. http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20942
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Prokop, Zbyněk Zdráhal, Zbyněk Kašparovský, Tomáš Lochman, Jan Mikeš, Vladimír Literáková, Petra
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím Molecular basis of cell and tissue regulations
issn	0021-9665
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Determination of capsidiol in tobacco cells culture by HPLC Determination of capsidiol in tobacco cells culture by HPLC Determination of capsidiol in tobacco cells culture by HPLC

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