About: Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa

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About: Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Directed evolution is a powerful tool widely used for elucidating the relationship between protein sequence, structure and function and also for producing proteins with altered or improved properties. The most useful approach in directed evolution is a random mutagenesis, which includes a lot of methods used to create diverse mutant libraries. LecB is the target gene we have modified via random mutagenesis, the gene coding PA-IIL lectin. This Pseudomonas aeruginosa soluble protein has been well-characterized. The random mutagenesis could help us to create a protein with interestingly modified specificity and affinity. After several trials we obtained an efficient method which introduces non-silent random mutations into the gene of our interest in sufficient rate. The mutant lectins with improved properties can be used in biotechnology and for bioanalytical purposes. Directed evolution is a powerful tool widely used for elucidating the relationship between protein sequence, structure and function and also for producing proteins with altered or improved properties. The most useful approach in directed evolution is a random mutagenesis, which includes a lot of methods used to create diverse mutant libraries. LecB is the target gene we have modified via random mutagenesis, the gene coding PA-IIL lectin. This Pseudomonas aeruginosa soluble protein has been well-characterized. The random mutagenesis could help us to create a protein with interestingly modified specificity and affinity. After several trials we obtained an efficient method which introduces non-silent random mutations into the gene of our interest in sufficient rate. The mutant lectins with improved properties can be used in biotechnology and for bioanalytical purposes. (en)
Title	Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa (en)
skos:prefLabel	Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa (en)
skos:notation	RIV/00216224:14310/09:00043043!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06030), S, Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Pokorná, Martina Wimmerová, Michaela Mrázková, Jana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	338072
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00043043
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	directed evolution; random mutagenesis in vitro; lectin PA-IIL; Pseudomonas aeruginosa (en)
http://linked.open.../riv/klicoveSlovo	Pseudomonas aeruginosa directed evolution random mutagenesis in vitro lectin PA-IIL
http://linked.open...ontrolniKodProRIV	[09FD379FBA1B]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomolecular centre
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Mrázková, Jana Pokorná, Martina Wimmerová, Michaela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14310

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