About: Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacrylate)-PGMA were prepared by single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNAs, degradation of intracellular PCR inhibitors and immunomagnetic separation of Salmonella cells were demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic acid (EDTA) immobilised on insoluble PGMA support and iminodiacetic acid (IDA) immobilised on magnetic P(HEMA-co-EDMA) microspheres were used as catalysts of plasmid DNA cleavage. Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacrylate)-PGMA were prepared by single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNAs, degradation of intracellular PCR inhibitors and immunomagnetic separation of Salmonella cells were demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic acid (EDTA) immobilised on insoluble PGMA support and iminodiacetic acid (IDA) immobilised on magnetic P(HEMA-co-EDMA) microspheres were used as catalysts of plasmid DNA cleavage. (en)
Title	Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications (en)
skos:prefLabel	Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications (en)
skos:notation	RIV/00216224:14310/09:00039058!RIV10-MZE-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1G57037), P(2B06053), P(2B08070), Z(AV0Z40500505), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	315727
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00039058
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzyme immobilization; PCR-ready DNA; amplification methods (en)
http://linked.open.../riv/klicoveSlovo	amplification methods P(HEMA-co-GMA) enzyme immobilization P(HEMA-co-EDMA) PGMA nonporous magnetic hydrophilic microspheres PCR-ready DNA
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[851D361A24FD]
http://linked.open...i/riv/nazevZdroje	FOOD RESEARCH INTERNATIONAL
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of molecular biology methods for the effective use of genetic sources of LAB and the characterisation of functional foods quality. Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production. New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	42
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Španová, Alena
http://linked.open...ain/vavai/riv/wos	000266197300014
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molecular basis of cell and tissue regulations
issn	0963-9969
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications

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