About: IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning. Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning. (en)
Title	IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH (en)
skos:prefLabel	IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH (en)
skos:notation	RIV/00216224:14310/09:00029986!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD204/08/H054), P(LC06034), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	318337
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029986
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CKI1; EMS mutagenesis; forward genetics (en)
http://linked.open.../riv/klicoveSlovo	CKI1 forward genetics EMS mutagenesis
http://linked.open...ontrolniKodProRIV	[632345FD8F26]
http://linked.open...v/mistoKonaniAkce	Mendel University of Agriculture and Forestry in
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	7th International Conference of Ph.D Students on Experimental Plant Biology %22News - what the plants told us%22
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular mechanisms of the cell proliferation and differentiation Regulation of morphogenesis of plant cells and organs
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Hejátko, Jan Kolouchová, Tereza
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2009-06-30 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
number of pages	28 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	978-80-7375-310-8
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH

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