About: Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.

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About: Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. (en)
Title	Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. (en)
skos:prefLabel	Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. (en)
skos:notation	RIV/00216224:14310/09:00029171!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1917), P(GD203/09/H046), P(LC06030), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Krejčí, Lumír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	352180
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/09:00029171
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Genome instability; recombination; DNA helicase; crossing over; Fanconi anemia (en)
http://linked.open.../riv/klicoveSlovo	Genome instability recombination DNA helicase Fanconi anemia crossing over
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[3689A13E11C5]
http://linked.open...i/riv/nazevZdroje	Genes Dev.
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomolecular centre Biochemistry on the crossroad from in silico to in vitro Resolution of replication-recombination DNA intermediates and its role in genomic instability
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Krejčí, Lumír Klein, Hannah Sung, Patrick Dray, E. Haber, J. E. Ira, Grzegorz Kramer, W. Papusha, A. Prakash, Rohit Satory, D. Scheller, J.
http://linked.open...ain/vavai/riv/wos	000262369700007
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	1549-5477
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.

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