About: Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides

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About: Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents and conserves the diffusion current. Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, the adsorptive stripping (AdS) procedure was favorably used. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents and conserves the diffusion current. Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, the adsorptive stripping (AdS) procedure was favorably used. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. (en)
Title	Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides (en)
skos:prefLabel	Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides (en)
skos:notation	RIV/00216224:14310/06:00016803!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1H-PK/42), P(IAA100040602), Z(AV0Z50040507), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Postbieglová, Irena Jelen, František Trnková, Libuše
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	465795
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00016803
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Reduction of adenine and cytosine in oligonucleotides; hanging mercury drop electrode (HMDE); elimination voltammetry with linear scan (EVLS); hetero-oligodeoxynucleotides (en)
http://linked.open.../riv/klicoveSlovo	hanging mercury drop electrode (HMDE) elimination voltammetry with linear scan (EVLS) hetero-oligodeoxynucleotides Reduction of adenine and cytosine in oligonucleotides
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[51A2EA887C48]
http://linked.open...i/riv/nazevZdroje	Electroanalysis
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and development of a new type of electrochemical biosensor for the detection of nucleotide sequences in DNA and of genotoxic agents in the environment. New approaches in electrochemical analysis of nucleic acids and oligonucleotides aimed at a ultrasensitive microdetection of DNA and DNA hybridization.
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Trnková, Libuše Jelen, František Postbieglová, Irena
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	1040-0397
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides

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