About: Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The piezoelectric quartz crystal operating in the active mode was used as a transducer for construction of immunosensors for determination polychlorinated biphenyls(PCB). An apparatus for flow-through measurements with piezosensors in pure organic phase was constructed. The anti-PCB polyclonal antibody was immobilized in an oriented way via protein A on a smooth gold surface. The antibody high cross-reactivity for 2,4,4'-trichlorobiphenyl, 4,4'-dichlorobiphenyl and 3,3,4,4'-tetrachlorbiphenyl allowed for a specific determination of these congeners in samples. PCBs were determined either directly or using competition with a tracer, synthesized from polystyrene particles by covalent attachment of 4,4'-dichlorobiphenyl (DCB). The direct piezoelectric immunosensor operated in phosphate buffer with 5% dimethylsulphoxide. It was possible to detect coplanar PCBs in the range 1-50ppm. A higher detection limit is compensated here by the low cost and short time of analysis, below 20 min. The piezoelectric quartz crystal operating in the active mode was used as a transducer for construction of immunosensors for determination polychlorinated biphenyls(PCB). An apparatus for flow-through measurements with piezosensors in pure organic phase was constructed. The anti-PCB polyclonal antibody was immobilized in an oriented way via protein A on a smooth gold surface. The antibody high cross-reactivity for 2,4,4'-trichlorobiphenyl, 4,4'-dichlorobiphenyl and 3,3,4,4'-tetrachlorbiphenyl allowed for a specific determination of these congeners in samples. PCBs were determined either directly or using competition with a tracer, synthesized from polystyrene particles by covalent attachment of 4,4'-dichlorobiphenyl (DCB). The direct piezoelectric immunosensor operated in phosphate buffer with 5% dimethylsulphoxide. It was possible to detect coplanar PCBs in the range 1-50ppm. A higher detection limit is compensated here by the low cost and short time of analysis, below 20 min. (en)
Title	Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases (en)
skos:prefLabel	Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases (en)
skos:notation	RIV/00216224:14310/06:00016802!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OBVTUO2003001), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Skládal, Petr Přibyl, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	492121
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00016802
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Biosensor; Toluene; Quartz crystal nanobalance (en)
http://linked.open.../riv/klicoveSlovo	Toluene Biosensor Quartz crystal nanobalance
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6269B12C8F18]
http://linked.open...i/riv/nazevZdroje	Sensors and Actuators B Chemical
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Imunosensors of CWA and BWA
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	113
http://linked.open...iv/tvurceVysledku	Skládal, Petr Přibyl, Jan Hepel, Maria
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0925-4005
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases

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