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Description
| - The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found.
- The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. (en)
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Title
| - Elimination voltammetry with linear scan as a new detection method for DNA sensors
- Elimination voltammetry with linear scan as a new detection method for DNA sensors (en)
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skos:prefLabel
| - Elimination voltammetry with linear scan as a new detection method for DNA sensors
- Elimination voltammetry with linear scan as a new detection method for DNA sensors (en)
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skos:notation
| - RIV/00216224:14310/05:00014629!RIV10-MSM-14310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1H-PK/42), P(GP525/04/P132), Z(AV0Z50040507), Z(MSM0021622412)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/05:00014629
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Kizek, René
- Trnková, Libuše
- Jelen, František
- Petrlová, Jitka
- Potěšil, David
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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