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  • The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found.
  • The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. (en)
Title
  • Elimination voltammetry with linear scan as a new detection method for DNA sensors
  • Elimination voltammetry with linear scan as a new detection method for DNA sensors (en)
skos:prefLabel
  • Elimination voltammetry with linear scan as a new detection method for DNA sensors
  • Elimination voltammetry with linear scan as a new detection method for DNA sensors (en)
skos:notation
  • RIV/00216224:14310/05:00014629!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1H-PK/42), P(GP525/04/P132), Z(AV0Z50040507), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika
  • 6-10
http://linked.open...vai/riv/dodaniDat
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http://linked.open...dnocenehoVysledku
  • 520102
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/05:00014629
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [F9B2A8F6B28A]
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  • SENSORS
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 5
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Kizek, René
  • Trnková, Libuše
  • Jelen, František
  • Petrlová, Jitka
  • Potěšil, David
http://linked.open...n/vavai/riv/zamer
issn
  • 1424-8220
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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