About: Elimination voltammetry with linear scan as a new detection method for DNA sensors

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. (en)
Title	Elimination voltammetry with linear scan as a new detection method for DNA sensors Elimination voltammetry with linear scan as a new detection method for DNA sensors (en)
skos:prefLabel	Elimination voltammetry with linear scan as a new detection method for DNA sensors Elimination voltammetry with linear scan as a new detection method for DNA sensors (en)
skos:notation	RIV/00216224:14310/05:00014629!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1H-PK/42), P(GP525/04/P132), Z(AV0Z50040507), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	6-10
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jelen, František Trnková, Libuše Adam, Vojtěch Kizek, René Potěšil, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	520102
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00014629
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides (en)
http://linked.open.../riv/klicoveSlovo	oligodeoxynucleotide sequence of nucleotides elimination voltammetry with linear scan linear sweep voltammetry mercury electrode DNA biosensor reduction of adenine and cytosine
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F9B2A8F6B28A]
http://linked.open...i/riv/nazevZdroje	SENSORS
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and development of a new type of electrochemical biosensor for the detection of nucleotide sequences in DNA and of genotoxic agents in the environment. Study of protection mechanisms for stress-induced by heavy metals
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Trnková, Libuše Jelen, František Petrlová, Jitka Potěšil, David
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	1424-8220
number of pages	17 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Elimination voltammetry with linear scan as a new detection method for DNA sensors

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