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  • A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors.The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane(BZE-DADOO) was immobilized on a monolayer of 11-mercaptomonoundecanoic acid (MUA) selfassembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the immobilized cocaine derivative was monitored with a mass sensitive piezoelectric quartz crystal (quartz crystal nanobalance; QCN). In the presence of an inhibiting substance in the sample, the binding of cholinesterase to the immobilized inhibitor was reduced. The decrease of the rate of mass change was proportional to the concentration of free inhibitor in the sample. This way the affinity sensor followed anti-cholinesterase toxicity and the enzyme activity of ChE was not addressed. An assay for detection of organophosphates (OP) was optimized.
  • A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors.The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane(BZE-DADOO) was immobilized on a monolayer of 11-mercaptomonoundecanoic acid (MUA) selfassembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the immobilized cocaine derivative was monitored with a mass sensitive piezoelectric quartz crystal (quartz crystal nanobalance; QCN). In the presence of an inhibiting substance in the sample, the binding of cholinesterase to the immobilized inhibitor was reduced. The decrease of the rate of mass change was proportional to the concentration of free inhibitor in the sample. This way the affinity sensor followed anti-cholinesterase toxicity and the enzyme activity of ChE was not addressed. An assay for detection of organophosphates (OP) was optimized. (en)
Title
  • Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor
  • Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor (en)
skos:prefLabel
  • Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor
  • Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor (en)
skos:notation
  • RIV/00216224:14310/05:00014127!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 542303
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/05:00014127
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Piezoelectric sensor; Quartz crystal microbalance; Acetylcholinesterase; Inhibitor (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [1A8CFB36274F]
http://linked.open...i/riv/nazevZdroje
  • Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 382
http://linked.open...iv/tvurceVysledku
  • Skládal, Petr
  • Přibyl, Jan
  • Halámek, Jan
  • Scheller, Frieder
  • Makower, Alexander
http://linked.open...n/vavai/riv/zamer
issn
  • 1618-2642
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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