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  • A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E.
  • A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. (en)
Title
  • Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese
  • Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese (en)
skos:prefLabel
  • Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese
  • Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese (en)
skos:notation
  • RIV/00216224:14310/05:00013899!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • Z(MSM0021622416)
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  • 520723
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  • RIV/00216224:14310/05:00013899
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  • Enterococcus; (GTG)5-PCR fingerprinting; bryndza (en)
http://linked.open.../riv/klicoveSlovo
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  • [CFAE1FA0D980]
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  • Sedláček, Ivo
  • Švec, Pavel
  • Seman, Milan
  • Swings, Jean
  • Vancanneyt, Marc
  • Lefebvre, Karen
  • Snauwaert, Cindy
http://linked.open...n/vavai/riv/zamer
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  • 14310
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