About: Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. (en)
Title	Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese (en)
skos:prefLabel	Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese (en)
skos:notation	RIV/00216224:14310/05:00013899!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622416)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Sedláček, Ivo Švec, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	520723
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00013899
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Enterococcus; (GTG)5-PCR fingerprinting; bryndza (en)
http://linked.open.../riv/klicoveSlovo	Enterococcus bryndza (GTG)5-PCR fingerprinting
http://linked.open...ontrolniKodProRIV	[CFAE1FA0D980]
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Seman, Milan Swings, Jean Vancanneyt, Marc Lefebvre, Karen Snauwaert, Cindy
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
http://localhost/t...ganizacniJednotka	14310

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