About: Application of Elimination Methods in Electrochemical Study of DNA

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak, the EVLS provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter. Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak, the EVLS provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter. (en)
Title	Application of Elimination Methods in Electrochemical Study of DNA Application of Elimination Methods in Electrochemical Study of DNA (en)
skos:prefLabel	Application of Elimination Methods in Electrochemical Study of DNA Application of Elimination Methods in Electrochemical Study of DNA (en)
skos:notation	RIV/00216224:14310/00:00003319!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany	1169
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GV204/97/K084)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Trnková, Libuše Kizek, René Dračka, Oldřich
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	704989
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/00:00003319
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Elimination of particular currents, elimination polarography/voltammetry, DNA, adsorbed species (en)
http://linked.open.../riv/klicoveSlovo	DNA Elimination of particular currents adsorbed species elimination polarography/voltammetry
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[5320849B48E2]
http://linked.open...i/riv/nazevZdroje	Journal of Biomolecular Structure and Dynamics
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Electrodes modified with nucleic acids and proteins.New tools in biochemical and biomedical research.
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Kizek, René Trnková, Libuše Dračka, Oldřich
issn	0739-1102
number of pages	1 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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