Attributes | Values |
---|
rdf:type
| |
Description
| - Minimal residual disease (MRD) detection is a well defined prognostic tool for response evaluation in hematological malignancies. Real time quantitative PCR (RQ-PCR) has become the settled and standardized method for MRD assessment in lymphoid disorders (van der Velden et al., Leukemia 2007). However, RQ-PCR based MRD detection relies on a relative quantification approach, since it requires a reference standard curve and an appropriate housekeeping gene for the normalization. Droplet Digital PCR (DD-PCR), applying the principle of limiting dilution of DNA and single molecule detection, allows reliable absolute tumor burden quantification at molecular level. In this study we performed a comparison of immunoglobulin heavy chain gene based MRD detection (IgH-MRD) performed by RQ-PCR and DD-PCR, to assess whether DD-PCR could overcome some RQ-PCR limitation and achieve superior sensitivity, robustness and accuracy.
- Minimal residual disease (MRD) detection is a well defined prognostic tool for response evaluation in hematological malignancies. Real time quantitative PCR (RQ-PCR) has become the settled and standardized method for MRD assessment in lymphoid disorders (van der Velden et al., Leukemia 2007). However, RQ-PCR based MRD detection relies on a relative quantification approach, since it requires a reference standard curve and an appropriate housekeeping gene for the normalization. Droplet Digital PCR (DD-PCR), applying the principle of limiting dilution of DNA and single molecule detection, allows reliable absolute tumor burden quantification at molecular level. In this study we performed a comparison of immunoglobulin heavy chain gene based MRD detection (IgH-MRD) performed by RQ-PCR and DD-PCR, to assess whether DD-PCR could overcome some RQ-PCR limitation and achieve superior sensitivity, robustness and accuracy. (en)
|
Title
| - Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM
- Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM (en)
|
skos:prefLabel
| - Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM
- Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM (en)
|
skos:notation
| - RIV/00216224:14110/13:00073962!RIV15-GA0-14110___
|
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14110/13:00073962
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - multiple myeloma; non-Hodgkin lymphoma; Minimal residual disease (MRD); PCR; Quantitative molecular analysis (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...iv/tvurceVysledku
| - Hájek, Roman
- Palumbo, Antonio
- Cortelazzo, Sergio
- Boccadoro, Mario
- Barberio, Davide
- Barbero, Daniela
- Bešše, Lenka
- Dani, Nadia
- Drandi, Daniela
- Ferrero, Simone
- Gambella, Manuela
- Genuardi, Elisa
- Ghione, Paola
- Guarona, Guglielmo
- Inghirami, Giorgio
- Ladetto, Marco
- Mantoan, Barbara
- Monitillo, Luigia
- Omedè, Paola
- Saraci, Elona
|
http://localhost/t...ganizacniJednotka
| |