About: Human Embryonic Stem Cells Are Capable of Executing G1/S Checkpoint Activation

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Embryonic stem cells progress very rapidly through the cell cycle, allowing limited time for cell cycle regulatory circuits that typically function in somatic cells. Mechanisms that inhibit cell cycle progression upon DNA damage are of particular importance, as their malfunction may contribute to the genetic instability observed in human embryonic stem cells (hESCs). In this study, we exposed undifferentiated hESCs to DNA-damaging ultraviolet radiation-C range (UVC) light and examined their progression through the G1/S transition. We show that hESCs irradiated in G1 phase undergo cell cycle arrest before DNA synthesis and exhibit decreased cyclin-dependent kinase two (CDK2) activity. We also show that the phosphatase Cdc25A, which directly activates CDK2, is downregulated in irradiated hESCs through the action of the checkpoint kinases Chk1 and/or Chk2. Importantly, the classical effector of the p53-mediated pathway, protein p21, is not a regulator of G1/S progression in hESCs. Embryonic stem cells progress very rapidly through the cell cycle, allowing limited time for cell cycle regulatory circuits that typically function in somatic cells. Mechanisms that inhibit cell cycle progression upon DNA damage are of particular importance, as their malfunction may contribute to the genetic instability observed in human embryonic stem cells (hESCs). In this study, we exposed undifferentiated hESCs to DNA-damaging ultraviolet radiation-C range (UVC) light and examined their progression through the G1/S transition. We show that hESCs irradiated in G1 phase undergo cell cycle arrest before DNA synthesis and exhibit decreased cyclin-dependent kinase two (CDK2) activity. We also show that the phosphatase Cdc25A, which directly activates CDK2, is downregulated in irradiated hESCs through the action of the checkpoint kinases Chk1 and/or Chk2. Importantly, the classical effector of the p53-mediated pathway, protein p21, is not a regulator of G1/S progression in hESCs. (en)
Title	Human Embryonic Stem Cells Are Capable of Executing G1/S Checkpoint Activation Human Embryonic Stem Cells Are Capable of Executing G1/S Checkpoint Activation (en)
skos:prefLabel	Human Embryonic Stem Cells Are Capable of Executing G1/S Checkpoint Activation Human Embryonic Stem Cells Are Capable of Executing G1/S Checkpoint Activation (en)
skos:notation	RIV/00216224:14110/10:00067228!RIV14-MSM-14110___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(NS10439), S, Z(AV0Z50390512), Z(AV0Z50390703), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hampl, Aleš Pospíšilová, Šárka Bárta, Tomáš Doležalová, Dáša Vinarský, Vladimír Dvořák, Petr Verner, Jan Holubcová, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	262331
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/10:00067228
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Human embryonic stem cells; DNA damage; Checkpoint activation; UVC; Cdc25A; p53 (en)
http://linked.open.../riv/klicoveSlovo	DNA damage p53 Human embryonic stem cells Cdc25A Checkpoint activation UVC
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0CF2204445E1]
http://linked.open...i/riv/nazevZdroje	Stem Cells
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of changes in genome and its expression in chronic lymphocytic leukemia patients during the disease development
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Hampl, Aleš Pospíšilová, Šárka Verner, Jan Bárta, Tomáš Doležalová, Dáša Vinarský, Vladimír Holubcová, Zuzana
http://linked.open...ain/vavai/riv/wos	000280746400004
http://linked.open...n/vavai/riv/zamer	Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie New biotechnologies, nanomaterials and stem cells for use in regenerative medicine Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	1066-5099
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/stem.451
http://localhost/t...ganizacniJednotka	14110

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