About: Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis     Goto   Sponge   NotDistinct   Permalink

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  • Microtubular and actin cytoskeletons were investigated by fluorescence and electron microscopy. Microtubules were detected by indirect immunofluorescence. Cytoplasmic microtubules were detected in interphase and a spindle with astral microtubules was seen in M-phase. The disintegration of cytoplasmic microtubules and migration of the nucleus to the bud before mitosis were characteristic features of the basidiomycetous yeast Malassezia pachydermatis. The visualisation of F-actin structures (patches, cables and cytokinetic rings) by fluorescence microscopy using both monoclonal anti-actin antibody and rhodamine-phalloidin failed, but actin was detected by electron microscopy with immunogold labelling. Clusters of gold particles indicating actin structures were detected at the plasma membrane of cells with unique cortical ultrastructural features characteristic of the genus Malassezia. A possible association of these with the actin cytoskeleton is suggested.
  • Microtubular and actin cytoskeletons were investigated by fluorescence and electron microscopy. Microtubules were detected by indirect immunofluorescence. Cytoplasmic microtubules were detected in interphase and a spindle with astral microtubules was seen in M-phase. The disintegration of cytoplasmic microtubules and migration of the nucleus to the bud before mitosis were characteristic features of the basidiomycetous yeast Malassezia pachydermatis. The visualisation of F-actin structures (patches, cables and cytokinetic rings) by fluorescence microscopy using both monoclonal anti-actin antibody and rhodamine-phalloidin failed, but actin was detected by electron microscopy with immunogold labelling. Clusters of gold particles indicating actin structures were detected at the plasma membrane of cells with unique cortical ultrastructural features characteristic of the genus Malassezia. A possible association of these with the actin cytoskeleton is suggested. (en)
Title
  • Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis
  • Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis (en)
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  • Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis
  • Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis (en)
skos:notation
  • RIV/00216224:14110/07:00020053!RIV11-GA0-14110___
http://linked.open...avai/riv/aktivita
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  • P(GA310/03/1195), P(GA310/06/0605)
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  • 1
http://linked.open...vai/riv/dodaniDat
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  • 433807
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  • RIV/00216224:14110/07:00020053
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  • actin; basidiomycetous yeasts; cytoskeleton; Malassezia pachydermatis; microtubules; ultrastructure (en)
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http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [77E9A39A5CC2]
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  • Cell Biology International
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  • 31
http://linked.open...iv/tvurceVysledku
  • David, Marek
  • Kopecká, Marie
  • Gabriel, Miroslav
http://linked.open...ain/vavai/riv/wos
  • 000244297300003
issn
  • 1065-6995
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  • 14110
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