About: Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve     Goto   Sponge   NotDistinct   Permalink

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  • Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 ľm) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence
  • Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 ľm) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence (en)
  • Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 ľm) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence (cs)
Title
  • Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve
  • Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve (en)
  • Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve (cs)
skos:prefLabel
  • Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve
  • Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve (en)
  • Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve (cs)
skos:notation
  • RIV/00216224:14110/02:00006844!RIV09-GA0-14110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA309/00/0407), Z(MSM 141100001)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 661310
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/02:00006844
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • neurotrophins; immunofluorescence; image analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [FC0B4E74301E]
http://linked.open...v/mistoKonaniAkce
  • 2002, Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • Progress in Basic, Applied, and Diagnostic Histochemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Dubový, Petr
  • Hradilová Svíženská, Ivana
  • Klusáková, Ilona
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Masarykova univerzita v Brně
https://schema.org/isbn
  • 80-210-2998-6
http://localhost/t...ganizacniJednotka
  • 14110
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