About: A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS     Goto   Sponge   NotDistinct   Permalink

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Description
  • The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the motor and primary sensory neurons for axon regeneration, we hypothesize different cellular and extracellular conditions in the dorsal and ventral roots based on various types of axons. Chondroitin sulfate proteoglycan (CSPG), fibronectin (FN), tenascin (TN) and thrombospondin (TSP) as well as BDNF, NGF and NT-3 were investigated by optimized indirect immunofluorescence method. Intensity of immunofluorescence staining was compared using the computer-assisted image analysis. The measurement of immunofluorescence intensity revealed that the endoneurial ECM of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. Two weeks after root transection, the immunofluorescence intensity for ECM molecules decreased in comparison to intact roots. In addition, a higher intensity for CSPG remained in the dors
  • The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the motor and primary sensory neurons for axon regeneration, we hypothesize different cellular and extracellular conditions in the dorsal and ventral roots based on various types of axons. Chondroitin sulfate proteoglycan (CSPG), fibronectin (FN), tenascin (TN) and thrombospondin (TSP) as well as BDNF, NGF and NT-3 were investigated by optimized indirect immunofluorescence method. Intensity of immunofluorescence staining was compared using the computer-assisted image analysis. The measurement of immunofluorescence intensity revealed that the endoneurial ECM of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. Two weeks after root transection, the immunofluorescence intensity for ECM molecules decreased in comparison to intact roots. In addition, a higher intensity for CSPG remained in the dors (en)
  • Dorsální a ventrální kořeny spinálních nervů obsahují aferentní a eferentní nervová vlákna, kolem kterých je vytvořena ECM rozdílného molekulárního složení. (cs)
Title
  • A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS
  • A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS (en)
  • Kvantitativní imunofluorescenční analýza ECM molekul (cs)
skos:prefLabel
  • A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS
  • A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS (en)
  • Kvantitativní imunofluorescenční analýza ECM molekul (cs)
skos:notation
  • RIV/00216224:14110/02:00005820!RIV09-GA0-14110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA309/00/0407), Z(MSM 141100001)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 637174
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/02:00005820
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • endoneurium; extracellular; matrix; neurotrophins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [AB1B8A241108]
http://linked.open...i/riv/nazevZdroje
  • Glia
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • Suppl. 1
http://linked.open...iv/tvurceVysledku
  • Dubový, Petr
  • Hradilová Svíženská, Ivana
  • Klusáková, Ilona
http://linked.open...ain/vavai/riv/wos
  • 000175586100188
http://linked.open...n/vavai/riv/zamer
issn
  • 0894-1491
number of pages
http://localhost/t...ganizacniJednotka
  • 14110
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