About: Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases     Goto   Sponge   NotDistinct   Permalink

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Description
  • 5-[(2-Nitrobenzyl) oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.
  • 5-[(2-Nitrobenzyl) oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes. (en)
Title
  • Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases
  • Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases (en)
skos:prefLabel
  • Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases
  • Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases (en)
skos:notation
  • RIV/00216208:11310/14:10271756!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
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  • I, P(GA14-04289S)
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  • 26
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 37420
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  • RIV/00216208:11310/14:10271756
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  • protective groups; photocaging; nucleotides; DNA polymerase; DNA (en)
http://linked.open.../riv/klicoveSlovo
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  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [663346066360]
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  • Angewandte Chemie - International Edition
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  • 53
http://linked.open...iv/tvurceVysledku
  • Hocek, Michal
  • Vaníková, Zuzana
http://linked.open...ain/vavai/riv/wos
  • 000338021600028
issn
  • 1433-7851
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/anie.201402370
http://localhost/t...ganizacniJednotka
  • 11310
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