About: Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS     Goto   Sponge   NotDistinct   Permalink

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  • The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs.
  • The main analytical benefit of this study is the development of methods enabling a rapid determination of total lipids of algae by lipidomic analysis and detailed identification and quantification of a complex mixture of natural TAGs by silver-LC/APCI-MS and NARP-LC/APCI-MS. Both types of chromatography can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:3 and 16:4 acids in the molecule. We conclude that the genus Chloromonas is a major producer of C16 PUFAs mostly contained in TAGs. Since more detailed studies in this field have been stymied by the shortage of 16:3 and 16:4 FAs, we decided to study the alga Chloromonas as a potential biotechnological source of C16 PUFAs. (en)
Title
  • Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS
  • Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS (en)
skos:prefLabel
  • Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS
  • Lipidomic profiling of snow algae by ESI-MS and silver-LC/APCI-MS (en)
skos:notation
  • RIV/00216208:11310/14:10218350!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GA14-00227S)
http://linked.open...iv/cisloPeriodika
  • April
http://linked.open...vai/riv/dodaniDat
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  • 26309
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  • RIV/00216208:11310/14:10218350
http://linked.open...riv/jazykVysledku
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  • NARP-LC/APCI-MS; Silver-LC/APCI-MS; Triacylglycerols; ESI; Lipidomics; Chloromonas pichinchae; Snow alga (en)
http://linked.open.../riv/klicoveSlovo
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  • GB - Spojené království Velké Británie a Severního Irska
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  • [41D8FB9D3C0C]
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  • Phytochemistry
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  • 100
http://linked.open...iv/tvurceVysledku
  • Nedbalová, Linda
  • Procházková, Lenka
  • Sigler, Karel
  • Rezanka, Tomas
http://linked.open...ain/vavai/riv/wos
  • 000333779700004
issn
  • 0031-9422
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.phytochem.2014.01.017
http://localhost/t...ganizacniJednotka
  • 11310
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