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  • Fused silica capillary columns of the internal diameter of 320 mu m were packed with the Nucleosil C18 stationary phase of 5 mu m using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied to study their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased. (C) 2013 Institute of Chemistry, Slovak Academy of Sciences
  • Fused silica capillary columns of the internal diameter of 320 mu m were packed with the Nucleosil C18 stationary phase of 5 mu m using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied to study their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased. (C) 2013 Institute of Chemistry, Slovak Academy of Sciences (en)
Title
  • Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
  • Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography (en)
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  • Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography
  • Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography (en)
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  • RIV/00216208:11310/14:10144018!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
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  • I, S, Z(MSM0021620857)
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  • 1
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  • 36131
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  • RIV/00216208:11310/14:10144018
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  • separation impedance; Van Deemter curve; slurry packing; high performance liquid chromatography (en)
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  • SK - Slovenská republika
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  • [F2E283F71AF7]
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  • Chemical Papers
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  • 68
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  • Bosáková, Zuzana
  • Coufal, Pavel
  • Franc, Martin
  • Sobotníková, Jana
  • Vojta, Jiří
http://linked.open...ain/vavai/riv/wos
  • 000324491200003
http://linked.open...n/vavai/riv/zamer
issn
  • 0366-6352
number of pages
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  • 10.2478/s11696-013-0422-9
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  • 11310
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