About: Applications of Capillary Electrophoresis in Enzyme Assays

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdfs:seeAlso	http://www.bestservis.eu/upload/file/Sbornik_2013.pdf
Description	In this work, we present several applications that manifest the potential of capillary electrophoresis in enzyme assays. Capillary electrophoresis was employed to perform offline and online assays of beta-N-acetylhexosaminidase for three different substrates. Offline assays were successfully utilized to monitor the enzyme reaction and to determine parameters such as pH and temperature optimum. The offline assay was also used to compare activities of the enzyme towards the natural and chromogenic substrates. Finally, an online enzyme assay was developed and proved to be highly beneficial for inhibitor screening. In this work, we present several applications that manifest the potential of capillary electrophoresis in enzyme assays. Capillary electrophoresis was employed to perform offline and online assays of beta-N-acetylhexosaminidase for three different substrates. Offline assays were successfully utilized to monitor the enzyme reaction and to determine parameters such as pH and temperature optimum. The offline assay was also used to compare activities of the enzyme towards the natural and chromogenic substrates. Finally, an online enzyme assay was developed and proved to be highly beneficial for inhibitor screening. (en)
Title	Applications of Capillary Electrophoresis in Enzyme Assays Applications of Capillary Electrophoresis in Enzyme Assays (en)
skos:prefLabel	Applications of Capillary Electrophoresis in Enzyme Assays Applications of Capillary Electrophoresis in Enzyme Assays (en)
skos:notation	RIV/00216208:11310/13:10172702!RIV14-GA0-11310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GBP206/12/G151), S, Z(MSM0021620857)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Doubnerová, Veronika Ryšlavá, Helena Křížek, Tomáš Velvarská, Romana Coufal, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	61846
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/13:10172702
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	electrophoretically mediated microanalysis; chitobiose; chitotriose; beta-N-acetylhexosaminidase; enzyme activity; capillary electrophoresis (en)
http://linked.open.../riv/klicoveSlovo	enzyme activity chitobiose chitotriose electrophoretically mediated microanalysis capillary electrophoresis beta-N-acetylhexosaminidase
http://linked.open...ontrolniKodProRIV	[F8504A4C94ED]
http://linked.open...v/mistoKonaniAkce	Jetřichovice, Czech Republic
http://linked.open...i/riv/mistoVydani	Ústí nad Labem
http://linked.open...i/riv/nazevZdroje	Sborník přednášek mezinárodní odborné konference XXXIII. Moderní Elektrochemické Metody Jetřichovice 20. - 24. května 2013
http://linked.open...in/vavai/riv/obor	CB
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http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Coufal, Pavel Doubnerová, Veronika Křížek, Tomáš Ryšlavá, Helena Velvarská, Romana
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2013-05-20 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Srsenová Lenka - BEST servis Ústí nad Labem
https://schema.org/isbn	978-80-905221-1-4
http://localhost/t...ganizacniJednotka	11310

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