About: Metallothioneins and Cancer

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metallothionein (MT) is a protein playing role in homeostasis of metal ions. Its relation with formation and progression of tumors is discussed. Here, we propose a new methodological approach for visualization of MT on PVDF membranes after dot- and electroblotting by using a commercial monoclonal antibody and polyclonalchicken antibodies. The optimized procedure was as follows. We dotted sample on membrane and let it to dry. Then, we blocked the membrane with BSA. After that, the membrane was incubated in chicken primary antibody, washed, and incubated in secondary antibody conjugated with horseradish peroxidase. To visualize, we used 3-aminoethyl-9-carbazole. Under these conditions, we estimated detection limit as 3 pg of MT per 1 microL. The optimal approach was further utilized for detection of MT level in 2 human fibroblast lines and in serum obtained from children with medulloblastoma. The results were in good agreement with differential pulse voltammetry –Brdicka reaction. Metallothionein (MT) is a protein playing role in homeostasis of metal ions. Its relation with formation and progression of tumors is discussed. Here, we propose a new methodological approach for visualization of MT on PVDF membranes after dot- and electroblotting by using a commercial monoclonal antibody and polyclonalchicken antibodies. The optimized procedure was as follows. We dotted sample on membrane and let it to dry. Then, we blocked the membrane with BSA. After that, the membrane was incubated in chicken primary antibody, washed, and incubated in secondary antibody conjugated with horseradish peroxidase. To visualize, we used 3-aminoethyl-9-carbazole. Under these conditions, we estimated detection limit as 3 pg of MT per 1 microL. The optimal approach was further utilized for detection of MT level in 2 human fibroblast lines and in serum obtained from children with medulloblastoma. The results were in good agreement with differential pulse voltammetry –Brdicka reaction. (en)
Title	Metallothioneins and Cancer Metallothioneins and Cancer (en)
skos:prefLabel	Metallothioneins and Cancer Metallothioneins and Cancer (en)
skos:notation	RIV/00216208:11130/09:5411!RIV10-MSM-11130___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA401990701), Z(MSM0021620813)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Eckschlager, Tomáš Figová, Katarína Hraběta, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 2. lékařská fakulta
http://linked.open...dnocenehoVysledku	325616
http://linked.open...ai/riv/idVysledku	RIV/00216208:11130/09:5411
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Metallothioneins; cancer marker; cancer cell chemoresistance; detection; capillary-zone-electrophoresis; performance liquid-chromatography; current stripping chronopotentiometry; differential-pulse polarography; mass-spectrometric detection; bioinorganic speciation analysis; curve resolution analysis; messenger-rna expression; mercury saturation assay; ki-67 antigen-expression (en)
http://linked.open.../riv/klicoveSlovo	messenger-rna expression performance liquid-chromatography detection mass-spectrometric detection Metallothioneins cancer cell chemoresistance curve resolution analysis differential-pulse polarography ki-67 antigen-expression cancer marker bioinorganic speciation analysis capillary-zone-electrophoresis mercury saturation assay current stripping chronopotentiometry
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[F1243CFD7EFD]
http://linked.open...i/riv/nazevZdroje	Current Protein & Peptide Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Adam, V. Eckschlager, Tomáš Hraběta, Jan Kizek, R. Figová, Katarína
http://linked.open...ain/vavai/riv/wos	000268918200004
http://linked.open...n/vavai/riv/zamer	The Molecular Basis of Childhood Malignancies and Therapeutic Applications
issn	1389-2037
number of pages	16 (xsd:int)
http://localhost/t...ganizacniJednotka	11130
is http://linked.open...avai/riv/vysledek of	Metallothioneins and Cancer Metallothioneins and Cancer Metallothioneins and Cancer Metallothioneins and Cancer

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