About: Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1078/S0171-9335(04)70023-0
Description	The available data concerning the subnucleolar localisation of the individual steps of precursor-ribosomal RNA (pre-rRNA) processing are ambiguous. According to in situ hybridisation studies, the late steps of pre-rRNA processing have been located into the granular component of the nucleolus, but factors engaged in these events were found being enriched in the dense fibrillar component. In this study, by utilisation of permeabilised human cells, we demonstrate that the newly synthesised, bromouridine-labelled pre-rRNAs reside at, or near, the sites of transcription. We provide evidence that processing of pre-rRNA occurs in permeabilised mammalian cells and that the incorporated bromouridine residues do not interfere with pre-rRNA maturation. Our results suggest that the maturation process of ribosomal RNA in permeabilised cells takes place at, or nearby, the site of transcription and that the processing complex is assembled during or early after the rRNA transcription. The available data concerning the subnucleolar localisation of the individual steps of precursor-ribosomal RNA (pre-rRNA) processing are ambiguous. According to in situ hybridisation studies, the late steps of pre-rRNA processing have been located into the granular component of the nucleolus, but factors engaged in these events were found being enriched in the dense fibrillar component. In this study, by utilisation of permeabilised human cells, we demonstrate that the newly synthesised, bromouridine-labelled pre-rRNAs reside at, or near, the sites of transcription. We provide evidence that processing of pre-rRNA occurs in permeabilised mammalian cells and that the incorporated bromouridine residues do not interfere with pre-rRNA maturation. Our results suggest that the maturation process of ribosomal RNA in permeabilised cells takes place at, or nearby, the site of transcription and that the processing complex is assembled during or early after the rRNA transcription. (en)
Title	Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription (en)
skos:prefLabel	Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription (en)
skos:notation	RIV/00216208:11120/00:00003755!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA302/99/0587), P(GA304/00/1481), P(VS96129), Z(AV0Z5039906)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Raška, Ivan Staněk, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	722966
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/00:00003755
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ribosomal RNA; processing; transcription; nucleolus; bromouridine (en)
http://linked.open.../riv/klicoveSlovo	transcription processing Ribosomal RNA bromouridine nucleolus
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[1CCC20F327E7]
http://linked.open...i/riv/nazevZdroje	European Journal of Cell Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional organization of the cell nucleus Dynamic structure and function in the cell nucleus Laboratory of gene expression
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Raška, Ivan Staněk, David Kiss, Tamás
http://linked.open...ain/vavai/riv/wos	000086411200006
http://linked.open...n/vavai/riv/zamer	The molecular and cellular basis of selected human diseases, mechanisms of normal and pathological function, principles of diagnosis and therapy
issn	0171-9335
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1078/S0171-9335(04)70023-0
http://localhost/t...ganizacniJednotka	11120

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