About: A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.jbc.org/content/286/16/14291.long
Description	The interferon-regulated transcription factor IRF-1 is predicted to be largely disordered outside of the DNA-binding domain. The recent identification of a functional binding interface for the E3-ubiquitin ligase CHIP within the major disordered domain of IRF-1 led us to ask whether this region might be employed more widely by regulators of IRF-1 function. Here we describe the use of peptide aptamer-based affinity chromatography coupled with mass spectrometry to define a multiprotein binding interface on IRF-1 (Mf2 domain; amino acids 106-140) and to identify Mf2-binding proteins from A375 cells. A selection of the Mf2 domain-binding proteins (NPM1, TRIM28, and YB-1) have been validated using in vitro and cell-based assays. Interestingly, although NPM1, TRIM28, and YB-1 all bind to the Mf2 domain, they have differing amino acid specificities, demonstrating the degree of combinatorial diversity and specificity available through linear interaction motifs. The interferon-regulated transcription factor IRF-1 is predicted to be largely disordered outside of the DNA-binding domain. The recent identification of a functional binding interface for the E3-ubiquitin ligase CHIP within the major disordered domain of IRF-1 led us to ask whether this region might be employed more widely by regulators of IRF-1 function. Here we describe the use of peptide aptamer-based affinity chromatography coupled with mass spectrometry to define a multiprotein binding interface on IRF-1 (Mf2 domain; amino acids 106-140) and to identify Mf2-binding proteins from A375 cells. A selection of the Mf2 domain-binding proteins (NPM1, TRIM28, and YB-1) have been validated using in vitro and cell-based assays. Interestingly, although NPM1, TRIM28, and YB-1 all bind to the Mf2 domain, they have differing amino acid specificities, demonstrating the degree of combinatorial diversity and specificity available through linear interaction motifs. (en)
Title	A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1 A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1 (en)
skos:prefLabel	A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1 A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1 (en)
skos:notation	RIV/00209805:_____/11:#0000145!RIV12-MSM-00209805
http://linked.open...avai/predkladatel	Masarykův onkologický ústav
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED2.1.00/03.0101), P(GAP304/10/0868), P(LC07017), Z(AV0Z50200510), Z(MO0FVZ0000604)
http://linked.open...iv/cisloPeriodika	16
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Vojtěšek, Bořivoj
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykův onkologický ústav
http://linked.open...dnocenehoVysledku	183917
http://linked.open...ai/riv/idVysledku	RIV/00209805:_____/11:#0000145
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	IRF-1; Mf2 domain; YB-1; TRIM28 (en)
http://linked.open.../riv/klicoveSlovo	IRF-1 Mf2 domain TRIM28 YB-1
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[52D1B03F3189]
http://linked.open...i/riv/nazevZdroje	The Journal of biological chemistry
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Regional Centre for Applied Molecular Oncology (RECAMO) The proteomics-based study on molecular mechanisms of early lymph node metastases formation in low-grade breast cancer Oncoproteomics Centre
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	286
http://linked.open...iv/tvurceVysledku	Vojtěšek, Bořivoj
http://linked.open...ain/vavai/riv/wos	000289556200052
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Informační zabezpečení krizového managementu pro oblast zdravotnictví
issn	0021-9258
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1074/jbc.M110.204602
is http://linked.open...avai/riv/vysledek of	A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1 A multiprotein binding interface in an intrinsically disordered region of the tumour suppressor protein Interferon Regulatory Factor-1

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