| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterial numbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the decline in the viability of MAP was statistically significant. Using culture, these threshold concentrations were 100 mu g/mL of vancomycin, 50-100 mu g/mL of nalidixic acid, and 100 mu g/mL of amphotericin B, respectively. Given that the two methods were found to be comparable, the PMA qPCR is a potentially more convenient and effective alternative to culture in detecting MAP.
- Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterial numbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the decline in the viability of MAP was statistically significant. Using culture, these threshold concentrations were 100 mu g/mL of vancomycin, 50-100 mu g/mL of nalidixic acid, and 100 mu g/mL of amphotericin B, respectively. Given that the two methods were found to be comparable, the PMA qPCR is a potentially more convenient and effective alternative to culture in detecting MAP. (en)
|
| Title
| - Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture
- Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture (en)
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| skos:prefLabel
| - Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture
- Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture (en)
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| skos:notation
| - RIV/00027162:_____/12:#0000882!RIV13-MZE-00027162
|
| http://linked.open...avai/predkladatel
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(ED0006/01/01), P(QH81065), Z(MZE0002716202)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/12:#0000882
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Mycobacterium avium subsp paratuberculosis; PMA qPCR; Culture; Viability; Paratuberculosis; In vitro (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Babák, Vladimír
- Králík, Petr
- Pavlík, Ivo
- Přibylová, Radka
- Kubíčková, L.
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1016/j.tvjl.2012.05.002
|
| is http://linked.open...avai/riv/vysledek
of | |
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