About: Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)     Goto   Sponge   NotDistinct   Permalink

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Description
  • A protocol is described for the adventitious shoot bud regeneration from leaf and stem explants in A. hippocastanum. Effect of different media (Heller, WPM, Rugini and DKW) and growth regulators (BA, TDZ and NAA) combinations on shoot organogenesis were examined. The highest shoot number and length was achieved on Rugini and WPM medium. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and the highest shoot bud number (9.7) were recorded in stem explants on WPM medium with 8.9 µM BA + 0.5 µM NAA. Generally, TDZ was less effective on shoot regeneration in stem and leaf explants compared to BA. Rooting of elongated shoots was significantly increased (68.1%) on full-strength WPM medium supplemented with 2.7 µM NAA. Rooted plantlets were acclimatized successfully under greenhouse conditions.
  • A protocol is described for the adventitious shoot bud regeneration from leaf and stem explants in A. hippocastanum. Effect of different media (Heller, WPM, Rugini and DKW) and growth regulators (BA, TDZ and NAA) combinations on shoot organogenesis were examined. The highest shoot number and length was achieved on Rugini and WPM medium. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and the highest shoot bud number (9.7) were recorded in stem explants on WPM medium with 8.9 µM BA + 0.5 µM NAA. Generally, TDZ was less effective on shoot regeneration in stem and leaf explants compared to BA. Rooting of elongated shoots was significantly increased (68.1%) on full-strength WPM medium supplemented with 2.7 µM NAA. Rooted plantlets were acclimatized successfully under greenhouse conditions. (en)
Title
  • Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)
  • Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.) (en)
skos:prefLabel
  • Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)
  • Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.) (en)
skos:notation
  • RIV/00027073:_____/13:#0001614!RIV14-MZE-00027073
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QH81101)
http://linked.open...iv/cisloPeriodika
  • 24 September 2013
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 104962
http://linked.open...ai/riv/idVysledku
  • RIV/00027073:_____/13:#0001614
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Rooting; Adventitious shoot regeneration; Stem explant; in vitro; Leaf explant (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [025581AABE8F]
http://linked.open...i/riv/nazevZdroje
  • Scientia Horticulturae
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 161
http://linked.open...iv/tvurceVysledku
  • Vlašínová, Helena
  • Mertelík, Josef
  • Šedivá, Jana
http://linked.open...ain/vavai/riv/wos
  • 000325595300033
issn
  • 0304-4238
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.scienta.2013.06.030
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