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Description
  • A collaborative study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative mustard protein determination in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false positive results or cross-reactivity in in-house validation with a broad range of food matrices range with no detectable mustard protein . All participants obtained the Mustard ELISA kit with standard operational procedures, the list of samples, samples and a protocol for recording test results. The study included 15 food samples and 2 spiked samples. The statistical tests (Cochran, Dixon and Mandel) and analysis of variance (ANOVA) were used for the evaluation of the collaborative study results. Repeatability and reproducibility limits as well as a limit of quantification (LOQ, 1.8 mg mustard proteins/kg) and a limit of detection (LOD, 0.5 mg mustard proteins/kg) for the kit were calculated.
  • A collaborative study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative mustard protein determination in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false positive results or cross-reactivity in in-house validation with a broad range of food matrices range with no detectable mustard protein . All participants obtained the Mustard ELISA kit with standard operational procedures, the list of samples, samples and a protocol for recording test results. The study included 15 food samples and 2 spiked samples. The statistical tests (Cochran, Dixon and Mandel) and analysis of variance (ANOVA) were used for the evaluation of the collaborative study results. Repeatability and reproducibility limits as well as a limit of quantification (LOQ, 1.8 mg mustard proteins/kg) and a limit of detection (LOD, 0.5 mg mustard proteins/kg) for the kit were calculated. (en)
Title
  • ELISA Kit for Mustard Protein Determination: Interlaboratory study
  • ELISA Kit for Mustard Protein Determination: Interlaboratory study (en)
skos:prefLabel
  • ELISA Kit for Mustard Protein Determination: Interlaboratory study
  • ELISA Kit for Mustard Protein Determination: Interlaboratory study (en)
skos:notation
  • RIV/00027022:_____/11:00000233!RIV12-MZE-00027022
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002702202)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 197277
http://linked.open...ai/riv/idVysledku
  • RIV/00027022:_____/11:00000233
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ELISA method; determination; mustard proteins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [A613C0F943E7]
http://linked.open...i/riv/nazevZdroje
  • Journal of AOAC International
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 94
http://linked.open...iv/tvurceVysledku
  • Brychta, Josef
  • Plicka, Jan
  • Baranda, Ana
  • Baršová, Soňa
  • Bulawová, Hana
  • Cuhra, Petr
  • Dvorská, Pavla
  • Gabrovská, Dana
  • Guisantes Del Barco, Jorge Antonio
  • Hanák, Petr
  • Iametti, Stefania
  • Jimenez, Esther
  • Kubík, Martin
  • Langerholc, Tomaz
  • Laukkanen, Marja-Leena
  • Malmheden Yman, Ingrid
  • Martinez Quesada, Jorge
  • Martinez de Maranon, Inigo
  • Pardo, Miguel Angel
  • Piknová, Lubica
  • Postigo Reza, Idoia
  • Rysová, Jana
  • Sunen Pardo, Esther
  • Takkinen, Kristina
  • Tomková, Květa
  • Čenčič, Avrelija
  • Štumr, František
http://linked.open...ain/vavai/riv/wos
  • 000289544500029
http://linked.open...n/vavai/riv/zamer
issn
  • 1060-3271
number of pages
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