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Description
  • Within the framework of the research project ELISA methods for the quantitative determination of allergenic substances in foodstuff and raw materials were developed. ELISA kits for allergenic proteins of milk (casein, beta-lactoglobulin and BSA) egg white proteins and mustard proteins were validated and collaborative studies were performed to prove the validation of the ELISA methods developed. Various methods of extraction were tested. The parameters as a limit of detection, as a limit of quantification, robustness, repeatability and accuracy were determined. A broad range of zero matrices for allergens were tested as well. The ELISA kits are suitable for the determination of allergens according to EU legislation Directive 2005/26/EC and Directive 2006/142/EC in the laboratories focused on this topic.
  • Within the framework of the research project ELISA methods for the quantitative determination of allergenic substances in foodstuff and raw materials were developed. ELISA kits for allergenic proteins of milk (casein, beta-lactoglobulin and BSA) egg white proteins and mustard proteins were validated and collaborative studies were performed to prove the validation of the ELISA methods developed. Various methods of extraction were tested. The parameters as a limit of detection, as a limit of quantification, robustness, repeatability and accuracy were determined. A broad range of zero matrices for allergens were tested as well. The ELISA kits are suitable for the determination of allergens according to EU legislation Directive 2005/26/EC and Directive 2006/142/EC in the laboratories focused on this topic. (en)
Title
  • Methods for the determination of allergenic substances in foods
  • Methods for the determination of allergenic substances in foods (en)
skos:prefLabel
  • Methods for the determination of allergenic substances in foods
  • Methods for the determination of allergenic substances in foods (en)
skos:notation
  • RIV/00027022:_____/09:00000059!RIV10-MZE-00027022
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002702202)
http://linked.open...iv/cisloPeriodika
  • 2009
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 325707
http://linked.open...ai/riv/idVysledku
  • RIV/00027022:_____/09:00000059
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • food allergy; ELISA methods; allergenic protein determination (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [FD338AF32F32]
http://linked.open...i/riv/nazevZdroje
  • Czech Journal of Food Sciences
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Dvorská, Pavla
  • Gabrovská, Dana
  • Hanák, Petr
  • Rysová, Jana
  • Tomková, Květa
  • Štumr, František
  • Šafářová, Petra
http://linked.open...ain/vavai/riv/wos
  • 000269005600117
http://linked.open...n/vavai/riv/zamer
issn
  • 1212-1800
number of pages
is http://linked.open...avai/riv/vysledek of
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