About: Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of different activation protocols on DNA integrity of porcine oocytes matured in vitro was analysed using the comet assay. The matured oocytes were then freed of cumulus cells and treated continuously or intermittently with a nitric oxide (NO) donor for 6 h. Standard activation with calcium ions (Ca2+) and culture without any treatment served as positive and negative controls, respectively. Exposure of oocytes to Ca2+ was associated with high activation efficiency, but decreased DNA integrity. The opposite was observed after continuous exposure to NO. Intermittent action of NO increased the activation rate, while the values of DNA damage remained at low levels. Our data suggest that an increased DNA instability could be the main reason compromising the further embryonic development of oocytes activated by the standard protocol. The intermittent treatment with NO thus represents a promising step to optimization of parthenogenetic activation of pig oocytes. The effect of different activation protocols on DNA integrity of porcine oocytes matured in vitro was analysed using the comet assay. The matured oocytes were then freed of cumulus cells and treated continuously or intermittently with a nitric oxide (NO) donor for 6 h. Standard activation with calcium ions (Ca2+) and culture without any treatment served as positive and negative controls, respectively. Exposure of oocytes to Ca2+ was associated with high activation efficiency, but decreased DNA integrity. The opposite was observed after continuous exposure to NO. Intermittent action of NO increased the activation rate, while the values of DNA damage remained at low levels. Our data suggest that an increased DNA instability could be the main reason compromising the further embryonic development of oocytes activated by the standard protocol. The intermittent treatment with NO thus represents a promising step to optimization of parthenogenetic activation of pig oocytes. (en)
Title	Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro (en)
skos:prefLabel	Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro (en)
skos:notation	RIV/00027014:_____/10:#0001178!RIV11-GA0-00027014
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA523/06/0295), P(QI101A166), Z(AV0Z50390512), Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Petr, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	255937
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/10:#0001178
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Comet assay, DNA integrity, Nitric oxide, Oocyte, Parthenogenesis, Pig (en)
http://linked.open.../riv/klicoveSlovo	Comet assay Oocyte Pig Parthenogenesis Nitric oxide DNA integrity
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[25094E6C9BD3]
http://linked.open...i/riv/nazevZdroje	Zygote
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Activation of pig oocytes using nitric oxide donors Biotechnology in pig husbandry and breeding
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Jílek, František Kratochvílová, Jana Petr, Jaroslav Sedmíková, Markéta Novotná, Božena
http://linked.open...ain/vavai/riv/wos	000274580200010
http://linked.open...n/vavai/riv/zamer	Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	0967-1994
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro Effect of different activation modes on DNA integrity of porcine M II oocytes matured in vitro

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