About: Microarrays and their Aplication in GMO Screening

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this research was developed in-house GMO chip for identification of four plants species, five genetically modified organisms (GMO) and three GMO screening elements. The main goal was to get signal after hybridization of labeled total genomic DNA on the chip. It was tested three ways of labeling templates using fluorescent Cy3/Alexa3 and Cy5/Alexa 5 colours, it was tested hybridization of PCR products, total genomic DNA and treated DNA on the chip. It was determinated sensitivity of developed chip in a way of dilution of hybridization teplate. The value of 25 ng DNA was find as a required amout of DNA, who give a significant signal. However, the signal after hybridization of total genomic DNA wasn’t significant. Thus, we propose another procedures for signal improvement of total genomic DNA. In this research was developed in-house GMO chip for identification of four plants species, five genetically modified organisms (GMO) and three GMO screening elements. The main goal was to get signal after hybridization of labeled total genomic DNA on the chip. It was tested three ways of labeling templates using fluorescent Cy3/Alexa3 and Cy5/Alexa 5 colours, it was tested hybridization of PCR products, total genomic DNA and treated DNA on the chip. It was determinated sensitivity of developed chip in a way of dilution of hybridization teplate. The value of 25 ng DNA was find as a required amout of DNA, who give a significant signal. However, the signal after hybridization of total genomic DNA wasn’t significant. Thus, we propose another procedures for signal improvement of total genomic DNA. (en) Tento příspěvek popisuje GMO chip pro identifikaci čtyřech rostlinných druhů, s pěti genetickými modifikacemi a tři GMO screeningové elementy. byly testovány tři postupy značení celogenomové DNA.byly použity fluorochromy Cy3/Alexa3 a Cy5/Alexa 5, hybridizace PCR prosuktů. Signálu bylo dosaženo při použití 25ug DNA. Specificita signálu však nebyla dostačující, proto jsme navrhli jiný způsob značení DNA. (cs)
Title	Microarrays and their Aplication in GMO Screening Microarrays and their Aplication in GMO Screening (en) Mikroaraye a jejich aplikace v GMO screeningu. (cs)
skos:prefLabel	Microarrays and their Aplication in GMO Screening Microarrays and their Aplication in GMO Screening (en) Mikroaraye a jejich aplikace v GMO screeningu. (cs)
skos:notation	RIV/00027006:_____/07:2485!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	56;61
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1B44068)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Ovesná, Jaroslava Hodek, Jan
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	433682
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/07:2485
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GMO; microarray; GMO chip (en)
http://linked.open.../riv/klicoveSlovo	GMO GMO chip microarray
http://linked.open...ontrolniKodProRIV	[54DBA9132595]
http://linked.open...v/mistoKonaniAkce	CR,VÚRV,v.v.i. Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	4th Advances in Molecular Biology : New Approaches in Plant Analysis
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Development and validation of methods for increasing food quality and food safety using molecular biology technologies in agreement with EU regulations.
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Ovesná, Jaroslava Hodek, Jan Demnerová, K.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2007-12-10 (xsd:date)
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	VÚRV,v.v.i.
https://schema.org/isbn	978-80-87011-12-6
is http://linked.open...avai/riv/vysledek of	Microarrays and their Aplication in GMO Screening Microarrays and their Aplication in GMO Screening Microarrays and their Aplication in GMO Screening

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