About: Immunological and physiological characteristics of the intestine in probiotic associated animals. Experimentally induced enterocolitis

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About: Immunological and physiological characteristics of the intestine in probiotic associated animals. Experimentally induced enterocolitis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=IAA5020101
Description	The objective of this grant proposal is to clarify and compare the role of probiotic bacteria as modulators of mucosal proliferation and diffrentiation in the intestine of monoassociated and germfree animals. The immunological study will concern membrane markers of intestinal lymphocytes which will be detected using flow cytometry. Cytokone expression will be evaluated by ELISA. A biochemical approach will provide information on intestinal brush border membrane activities, tryptic activity and butyrate production by microflora. Our project will include experimental colitis induced in BALB/c and SCID mice by dextran sulfate treatment. The intestinal inflammation will be influenced by administration of bacteria with probiotic effects in order to diminish severity of colonocyte injury. Immunohistochemical and biochemical approach enables to compare changes in degree of inflammation. Expected results will contribute to our knowledge of inflammatory processes in the intestine. (en) Cílem našeho grantového projektu je studovat roli probiotických bakterií jako modulátorů proliferace a diferenciace buněk střevní sliznice u monoasociovaných a bezmikrobních zvířat. Změny v expresi membránových znaků střevních lymfocytů (lamina propria, Peyerových plátů, mezenteriálních uzlin) budeme sledovat průtokovou cytometrií, hladiny cytokinů budeme vyhodnocovat metodou ELISA. Stanovíme aktivity enzymů kartáčového lemu enterocytů, tryptickou aktivitu a produkci butyrátu mikroflorou. Dextransulfátem vyvoláme enterokolitidu u imunokompetentních BALB/c a imunodeficitních SCID myší a potom budeme studovat vliv probiotik na střevní zánět s cílem zmírnit poškození mukózy. Imunochemické a biochemické přístupy nám umožní porovnat změny ve stupni zánětu. Předpokládáme, že získané nálezy obohatí naše znalosti o zánětlivých procesech ve střevě.
Title	Immunological and physiological characteristics of the intestine in probiotic associated animals. Experimentally induced enterocolitis (en) Imunologické a fyziologické charakteristiky střeva zvířat osazených probiotickými bakteriemi. Vliv probiotik na experimentální enterokolitidu
skos:notation	IAA5020101
http://linked.open...avai/cep/aktivita	Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/IAA5020101/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/IAA5020101/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2007-09-26 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	1715177
http://linked.open...ai/cep/hlavniObor	FN - Epidemiologie, infekční nemoci a klinická imunologie
http://linked.open...hodnoceniProjektu	U - Uspěl podle zadání, tj. byly splněny cíle a jeho předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	Experimental induced enterocolitis; probiotic bacteria; intestine; enterocyte brush border enzymes; lactase; sucrase; blucoamylace; dipeptidyl peptidase IV; gamma-glutamyltranspeptidase; alkaline phosphatese; immunoglobulins; mouse; piglet (en)
http://linked.open...ep/partnetrHlavni	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	2 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	30 (xsd:int)
http://linked.open...enychVysledkuVRIV	30 (xsd:int)
http://linked.open...okUkonceniPodpory	2004
http://linked.open...okZahajeniPodpory	2001
http://linked.open...iciPoslednihoRoku	2005
http://linked.open...atUdajeProjZameru	2005
http://linked.open.../vavai/cep/soutez	SAV0-AB2001
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open.../cep/vedlejsiObor	EC - Imunologie FE - Ostatní obory vnitřního lékařství
http://linked.open...jektu+dodavatelem	Zavedli jsme myší model ulcerózní kolitidy a zjistili jsme, že podáváním některých bakterií je možno zánět zmírnit. Zjistili jsme, že u selat monokolonizace E. coli O83 a O86 stimuluje enzymy kartáčového lemu enterocytů a hladinu TNF-alfa v séru. (cs) We established a mice model of ulcerative colitis and we found that applying of certain bacteria reduce the inflammation. E. coli O83 and O86 stimulated activities of enterocyte brush-border enzyme activities and the level of TNF-alpha in sera. (en)
http://linked.open...tniCyklusProjektu	ZBBKU
http://linked.open.../cep/klicoveSlovo	alkaline phosphatese blucoamylace dipeptidyl peptidase IV enterocyte brush border enzymes gamma-glutamyltranspeptidase immunoglobulins intestine lactase mouse probiotic bacteria sucrase Experimental induced enterocolitis
is http://linked.open...vavai/riv/projekt of	Cytostatic and immunomobilizing activities of polymer-bound drugs: experimental and first clinical data Clinical Implications of N-(2-Hydroxypropyl)Methacrylamide Copolymers. Antibody-targeted HPMA copolymer-bound anthracycline antibiotics. Experimentally Induced Gluten Enteropathy and Protective Effect of Epidermal Growth Factor in Artificially Fed Neonatal Rats. Epitopes of calreticulin recognised by IgA autoantibodies from patients with hepatic and coeliac disease. Biochemical Intestinal Parameters in Germ-free Minipigs and Rats and in Ex-germ-free Minipigs and Rats Monoassociated with Escherichia coli Interaction of Mucosal Microbiota with the Innate Immune System Effect of Bacteroides ovatus on severity of experimentally induced chronic intestinal inflammation Effect of bacterial monoassociation on brush-border enzyme activities in ex-germ-free piglets: comparison of commensal and pathogenic E. coli strains Gluten and chronic diseases: Inflammatory activity of gluten The Role of Microflora in the Development of Intestinal Inflammation: Acute and Chronic Colitis Induced by Dextran Sulfate in Germ-Free and Conventionally Reared Immunocompetent and Immunodeficient Mice. Differential Effect of Bacillus firmus on Immune Response and Enterocyte Brush-Border Levels in BALB/c and B10.BR Mice. Effect of non-pathogenic E.coli on brushborder enzymes and immune responses in gnotobiotic pigs. Specific antibody and immunoglobulin responses after intestinal colonization of germ-free piglets with non-pathogenic Escherichia coli 086 Specific antibody and immunoglobulin responses after intestinal colonization of germ-free piglets with non-pathogenic Escherichia coli O86. Effect of monoassociation with probiotic strain Bifidobacterium bifidum on enterocyte brush-border enzymes in gnotobiotic mice. Mucosaů Immunity: Its Role in Defense and Allergy. Plasma lipopolysaccharide level and enterocyte brush border enzymes in gnotobiotic piglets infected with Salmonella typhimurium. Maternal Milk Reduces Severity of Necrotizing Enterocolitis and Increases Intestinal IL-10 in a Neonatal Rat Model. Vitamin A deficiency leads to severe functional disturbance of the intestinal epithelium enzymes associated with diarrhoea and increased bacterial translocation in gnotobiotic rats. Inactivated Bacillus firmus affects intestinal brush border enzymes in germ-free and conventional mice. Inactivated Bacillus Firmus affects intestinal immune responses and small intestinal brush border enzymes in germ-free and conventional mice. HPMA copolymers, drug carriers, doxorubicin, drug targeting, drug delivery, cytotoxicity Influence of microflora in development of intestinal inflammation induced by dextran sodium suofate in immunocompetent (BALB/c) and immunodeficient (SCID) mice. Influence o microflora in development of intestinal inflammation induced by dextran sodium sulfate in immunocompetent (BALB/c) and immunodeficient (SCID) mice. Influence of probiotic bacteria on the severity of experimentally induced intestinal inflammation in mice. Influence of microflora in development of intestinal inflammation induced by dextran sodium sulfate in mice. The proinflammatory cytokine IL-18 is constitutively expressed in the rat intestinal epithelial cell line IEC-6. Bifidobacterium bifidum reduces the development of experimentally induced colitis in mice Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases
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