About: Cellular and molecular basis of oogenesis fertilization and embryonic development in mammals

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=GV524/96/K162
Description	Results derived from the experiments involved in the proposed grant will potentially shed light not only on biochemical regulation of growth and maturation of mammalian oocytes, but it will allow to develop novel protocols which allow to include growing oocytes in embryo transfer program. Moreover, the role of cell surface proteins, acrosome proteins and tyrosine phosphorylated proteins of mammalian spermatozoa during capacitation, their motility and binding to oocytes will be elucidated upon animal andhuman model. We expect to identify a battery of genes whose expression increases during preimplantation development of bovine embryos. The precise comparison with zygotic gene activation in the mouse will allow to identify the best model system for the human. The mesoderm specification in the mouse embryos will be used to determine mechanism underlying the cellular differentiation and tissue patterning during early vertebrate embryogenesis. Taken together, the project, based upon the recent methodology (en) Experimentální výsledky, získané při řešení první části předkládaného grantu, poskytnou nejen prioritní údaje o mechanismu regulace růstu a zrání oocytů na biochemické a molekulární úrovni, ale zároveň umožní vypracovat metodiku, která včlení rostoucí oocyty do programu přenosu embryí. Druhá část grantu objasní specifický význam povrchových i akrozomálních proteinů a bílkovin fosforylovaných na tyrozínu savčích spermií pro průběh kapacitace, jejich motilitu a vazbu na oocyt. Třetí část grantu si kladza cíl identifikovat soubor genů, které jsou exprimovány v průběhu časného embryonálního vývoje u skotu. Současně bude na myším modelu prostudován mechanismus diferenciace buněk mezodermu v průběhu embryogeneze. Celý projekt, který je založen na současných metodických přístupech buněčné a molekulární biologie, bude řešit významný soubor otázek reprodukční biologie se zřetelnou orientací na jejich biomedicínské aspekty.
Title	Cellular and molecular basis of oogenesis fertilization and embryonic development in mammals (en) Molekulární a buněčné základy oogeneze oplození a embryonálního vývoje savců
skos:notation	GV524/96/K162
http://linked.open...avai/cep/aktivita	Comprehensive projects
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/GV524%2F96%2FK162/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/GV524%2F96%2FK162/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2002-11-19 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	877076
http://linked.open...ai/cep/hlavniObor	EB - Genetika a molekulární biologie
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open.../cep/klicovaSlova	Neuvedeno. (en)
http://linked.open...ep/partnetrHlavni	Ústav živočišné fyziologie a genetiky AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	3 (xsd:int)
http://linked.open.../pocetVysledkuRIV	86 (xsd:int)
http://linked.open...enychVysledkuVRIV	86 (xsd:int)
http://linked.open...iciPoslednihoRoku	2002
http://linked.open...atUdajeProjZameru	2002
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open.../cep/vedlejsiObor	EI - Biotechnologie a bionika
http://linked.open...jektu+dodavatelem	Spolupráce několika špičkových pracovišť na řešení projektu přinesla množství zajímavých výsledků, z nichž některé lze hodnotit jako prioritní. Výsledky výzkumu mechanismů oplození a embryonálního vývoje otevírají perspektivy dalšího bádání v této oblast (cs)
http://linked.open...tniCyklusProjektu	ZBBBBKU
is http://linked.open...vavai/riv/projekt of	Genetic rescue of an endangered mammal by cross-species nuclear transfer using post mortem somatic cells Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer. Is obesity infectious? A chemoenyzmatic route to mannosamine derivatives bearing different N-acyl groups Monoclonal antibodyes reacting against capacitated but not with freshly ejaculated boar spermatozoa. Preparation of fluorescein-labelled and biotinylated derivatives of polysaccharides for lectin-saccharide binding studies. Effects of follicle-stimulating hormone, bovine somatotropin and okadaic acid on cumulus expansion and nuclear maturation of Blue fox (Alopex lagopus) oocytes in vitro. Saccharide-Binding Properties of Boar AQN Spermadhesins and DQH Sperm Surface Protein. Regulation of Protein Tyrosine Phosphorylation in Boar Sperm Through a cAMP-Dependent Pathway. Determination of complete covalent structure of the major glycoform DQH sperm surface protein, a novel trypsin resistant boar seminal plasma O-glycoprotein related to pB1 protein Determination of the complete covalent structure of the major glycoform of DQH sperm surface protein, a novel trypsin-resistant boar seminal plasma O-glycoprotein related to pBl protein. Lectin-like properties of boar spermadhesis. Preparation of Biotinylated and FITC-Labelled Phosphorylcholine Poly(acrylamide) Derivatives and Their Application for Protein Ligand-Binding Studies. Butyrolactone I Reversibly Inhibits Meiotic Maturation of Bovine Oocytes, Without Influencing Chromosome Condensation Activity. Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence. High developmental competence of cattle oocytes maintained at the germinal vesicle stage for 24 hours in culture by specific inhibition of MPF kinase activity Influence of dietary fat source and copper supplementation on broiler performance, fatty acid profile of meat and depot fat, and on cholesterol content in meat Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes. Monoclonal Antibody to Human Sperm Acrosomal Protein. Monoclonal antibodies against mammalian sperm proteins as a tool for the study of fertilization. Resumption of meiosis induced by meiosis-activating sterol (MAS) has a different signal transduction pathway than spontaneous resumption of meiosis in denuded mouse oocytes cultured in vitro. Nuclei of nonviable ovine somatic cells develop into lambs after nuclear transplantation New monoclonal antibody to human apolipoprotein J. Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction Dynamic and role of cytoskeletal proteins in mammalian sperm cells. RNA differential display patterns from pig granulosa cells. Automated Recording of RNA Differential Display Patterns from Pig Granulosa Cells. Antibody aganist intra - acrosomal sperm protein 28 kD as a tool for evaluation of acrosomal integrity in bull spermatozoa. Association of protein kinase a type with detergent - resistant structures of mammalian sperm cells. Accumulation of the Proteolytic Marker Peptide Ubiquitin in the Trophoblast of Mammalian Blastocysts. Activation of pig and cattle oocytes by butyrolactone I: morphological and biochemical study. Alternative sources of gametes. Stimulatory Action of Intrafollicular Factors on Cumulus Expansion of Porcine Oocyte-Cumulus Cell Complex. Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm protein as marker for testing sperm damage. Immobilization of L-glyceryl phosphorylcholine: isolation of phosphorylcholine-binding proteins from seminal plasma. Isolation of non-heprin-binding and heparin-binding proteins of boar prostate. Interplay between cdc2 kinase and MAP kinase pathway during maturation of mammalian oocytes. Nonradioactive RNA Differential Display from Pig Membrana Granulosa Cells. Spermadhesins of the AQN and AWN families, DQH sperm surface protein and HNK protein in the heparin-binding fraction of boar seminal plasma. Checkpoint control of the G2/M phase transition during the first mitotic cycle in mammalian eggs Changes in immunochemical localization of cytoskeletal proteins in human and boar spermatozoa Aggregated Forms of Heparing-Binding and Non-Heparing-Binding Proteins of Boar Seminal Plasma and their Binding Properties. Cell-Cycle Control and Oocyte Maturation:Review of Literature. Activation of bovine oocytes by combination of Ca2+ release and inhibition of cdc2 kinase activity. Binding properties of boar seminal plasma proteins and their aggregated forms. Changes in Immunochemical Localization of Cytoskeletal Proteins in Human and Boar Spermatozoa before and after Acrosome Reaction. Characterization of Boar Spermadhesins by Monoclonal and Polyclonal Antibodies and Their Role in Binding to Oocytes. Embryo cloning by nuclear transfer: experiences in sheep The influence of mammalian sperm acrosomal reaction by cytoskeletal proteins N/A Heparin-binding and non-heparing-binding proteins of boar seminal plasma and their aggregated forms. Interaction of boar spermadhesins with acidic polysaccharides. Nucleolar Ultrastructure in Bovine Nuclear transfer Embryos. O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells. Aggregated and Monomeric Forms of Proteins in Boar Seminal Plasma: Characterization and Binding Properties. Cytoskeletal proteins and their changes after acrosomal reaction Cytoskeletální proteiny a jejich změny po akrozomální reakci spermií v přítomnosti specifických inhibitorů Cytoskeletální proteiny savčích spermií a jejich změny po akrozomální reakci v přítomnosti specifických inhibitorů D-penicillamine and granulosa cells can effectively extend the fertile life span of bovine frozen-thawed spermatozoa in vitro: Effect on fertilization and polyspermy. Levels and Interactions of p27, Cyclin D3, and CDK4 During the Formation and Maintenance of the Corpus Luteum in Mice. Inhibition of the antibody responses to rat blood transfusion antigens in mice by boar seminal immunosuppressive fraction. Sperm acrosomal proteins and their changes after acrosomal reactions by specific inhibitors Sperm Surface Proteins in Mammalian Fertilization. Sperm Surface in Mammalian Fertilization. Využití monoklonálních protilátek proti proteinům lidských spermií k diagnostice patologie spermií a výběru vhodné metody fertilizace v asistované reprodukci. Changes in Immunocytochemical Localization of Cytoskeletal Proteins in Boar Spermatozoa after Acrosome Reaction Induced by Specific Inhibitors Comparative Aspects of Follicular Development, Follicular and Oocyte Maturation and Ovulation in Cattle and Pigs. Changes in immunocytochemical localization of cytoskeletal proteins in boar spermatozoa after acrosome reaction induced by specific cytoskeletal inhibitors. Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin b and MAP kinase activities. A role for map kinase in nuclear envelope assembly.

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