About: Multidimensional separation and detection of biomolecules by high-performance capillary electromigration methods

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About: Multidimensional separation and detection of biomolecules by high-performance capillary electromigration methods Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=GA203/05/2539
Description	A new instrumentation and methodology of high-performance capillary electromigration methods will be developed, with the aim to improve and enlarge applicability of these methods for high-sensitivity analysis and characterization of biomolecules, e.g. peptide and protein hormones, immunomodulators, enzymes and their inhibitors, potentially applicable in human and veterinary medicine. Capillary electrokinetic analyzer of a new generation will be constructed, which will be equipped with multidimensionaldetection system, composed of spectrophotometric detector, double contactless conductivity detector (CLCD) and fluorescence detector with laser induced fluorescence (LIF). CLCD allows analysis of ionogenic compounds non-possessing chromophores and fluorophores. LIF detector with excitation wavelength 266 nm will substantially increase the sensitivity of analysis of peptides and proteins containing aromatic amino acids due to their native fluorescence, i.e. without need of their derivatization. In (en) Podstatou projektu bude vývoj nové instrumentace a metodiky vysokoúčinných kapilárních elektroseparačních metod, s cílem zdokonalit a rozšířit aplikační možnosti těchto metod pro vysoce citlivou analýzu a charakterizaci biologicky aktivních látek, např. peptidových a bílkovinných hormonů, imunomodulátorů, enzymů a jejich inhibitorů, potenciálně využitelných v humánní a veterinární medicíně. Bude vyvinut kapilární elektrokinetický analyzátor nové generace vybavený multidimenzionálním detekčním systémem, skládajícím se ze spektrofotometrického detektoru, dvojitého bezkontaktního vodivostního detektoru a fluorescenčního detektoru s laserem indukovanou fluorescencí (LIF). Bezkontaktní vodivostní detektor umožní analýzu ionogenních látek neobsahujících chromofory nebo fluorofory. LIF detektor s excitační vlnovou délkou 266 nm mnohonásobně zvýší citlivost analýzy peptidů a bílkovin obsahujících aromatické aminokyseliny na bázi jejich nativní fluorescence, tj. bez nutnosti jejich derivatizace. V
Title	Multidimensional separation and detection of biomolecules by high-performance capillary electromigration methods (en) Multidimenzionální separace a detekce biomolekul vysokoúčinnými kapilárními elektromigračními metodami
skos:notation	GA203/05/2539
http://linked.open...avai/cep/aktivita	Standard projects
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/GA203%2F05%2F2539/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/GA203%2F05%2F2539/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2008-12-16 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	71263339
http://linked.open...ai/cep/hlavniObor	CB - Analytická chemie, separace
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	electromigration methods; multidimensional separation; detection; biomolecules (en)
http://linked.open...ep/partnetrHlavni	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	2 (xsd:int)
http://linked.open.../pocetVysledkuRIV	60 (xsd:int)
http://linked.open...enychVysledkuVRIV	60 (xsd:int)
http://linked.open...lneniVMinulemRoce	2007-05-02 (xsd:date)
http://linked.open.../prideleniPodpory	http://linked.opendata.cz/resource/domain/vavai/cep/prideleniPodpory/203%2F05%2F2539
http://linked.open...iciPoslednihoRoku	2007
http://linked.open...atUdajeProjZameru	2008
http://linked.open.../vavai/cep/soutez	SGA02005GA-ST
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open...ep/ukonceniReseni	2007-12-31 (xsd:date)
http://linked.open.../cep/vedlejsiObor	CF - Fyzikální chemie a teoretická chemie CE - Biochemie
http://linked.open...ep/zahajeniReseni	2005-01-01 (xsd:date)
http://linked.open...jektu+dodavatelem	Projekt přinesl nové výsledky v oblasti instrumentace, metodiky a teorie vysokoúčinných kapilárních elektromigračních metod a rozšířil možnosti jejich využití pro analýzu, purifikaci a fyzikálně chemickou a biochemickou charakterizaci aminokyselin, pepti (cs) Project has brought new results in the area of instrumentation, metodology and theory of high-performance capillary electromigration methods and enlarged their applicability to analysis, purification and physiochemical and biochemical characterization of (en)
http://linked.open...tniCyklusProjektu	ZBKU
http://linked.open.../cep/klicoveSlovo	electromigration methods detection multidimensional separation
is http://linked.open...vavai/riv/projekt of	Recent developments in CE and CEC of peptides Determination of binding constants of valinomycin complexes with ammonium and alkali metal ions by capillary affinity electrophoresis From micro- to macro: Conversion of capillary electrophoretic separations of biomolecules and bioparticles to preparative free-flow electrophoresis scale Mapping the peptide and protein immune response in the larvae of the fleshfly Sarcophaga bullata Synthesis of guanidino analogues of PMPDAP and their immunobiological activity Kohlrausch regulating function and other conservation laws in electrophoresis Posttranslational modifications of collagen studied by off-line coupling of HPLC and CE Eigenmobilities in background electrolytes for CZE. V. Intensity (amplitudes) of system peaks Oscillating electrolytes Determination of limiting mobilities and dissociation constants of 21 amino acids by capillary zone electrophoresis at very low pH Capillary electrophoresis of peptides and proteins with plug of Pluronic gel Capillary electromigration methods for the study of collagen Matrices for capillary gel electrophoresis--a brief overview of uncommon gels Separation of poly(amidoamine) (PAMAM) dendrimer generations by dynamic coating capillary electrophoresis Separation and investigation of structure-mobility relationship of gonadotropin-releasing hormones by capillary zone electrophoresis in conventional and isoelectric acidic background electrolytes Voltage-assisted capillary LC of peptides using monolithic capillary columns prepared by ring-opening metathesis polymerization Proteins of Insoluble Matrix of Avian (Gallus Gallus) Eggshell Study of posttranslational non-enzymatic modifications of collagen using capillary electrophoresis/mass spectrometry and high performance liquid chromatography/mass spectrometry Capillary electrochromatography of proteins and peptides Analysis, separation and investigation of structure-mobility relationships of biopeptides by capillary zone electrophoresis Recent advances in capillary electrophoresis of peptides and proteins Investigation of structure-mobility relationships and estimation of secondary structure of biopeptides by capillary zone electrophoresis Separation of synthetic oligopeptides and terbutaline enantiomers by capillary zone electrophoresis with electroosmotic flow controlled by radial electric field Capillary electrophoresis assay for D-serine Physicochemical characterization of peptides and proteins by capillary electromigration methods System peaks in micellar electrophoresis: 1. Utilization of system peaks for determination of critical micelle concentration PeakMaster - A freeware simulator of capillary zone electrophoresis Investigation of the effect of ionic strength of Tris-acetate background electrolyte on electrophoretic mobilities of mono-, di-, and trivalent organic anions by capillary electrophoresis Limitní pohyblivosti a disociační konstanty kationtových forem vybraných aminokyselin stanovované CZE v kyselé pH oblasti Determination of acid-base dissociation constants of amino- and guanidinopurine nucleotide analogues and related compounds by capillary zone electroforesis Recent developments in capillary electrophoresis and capillary electrochromatography of peptides Physicochemical characterization of biopeptides by capillary electrophoresis in background electrolytes within a broad pH range (1.4-12.0) Determination of D-serine by capillary electrophoresis Capillary electrophoresis method for the determination of D-serine and its application for monitoring of serine racemase activity Recent applications of conductivity detection in capillary and chip electrophoresis Determination of purity degree and counter-ions content in lecirelin preparations by capillary zone electrophoresis and capillary isotachophoresis Synthesis and properties of 2-guanidinopurines Determination of pKa values of diastereomers of phosphinic pseudopeptides by CZE Kapilární elektroseparační analyzátor s multidimenzionální detekcí Control of EOF in CE by different ways of application of radial electric field Separation and characterization of peptide and pseudopeptide stereoisomers by capillary electrophoresis Characterization of acid-base properties of polyprotic compounds by capillary electrophoresis Capillary electrophoretic study of interactions of ammonium and alkali metal ions with valinomycin Separation and characterization of peptides by capillary electrophoresis in strongly acidic/basic background electrolytes Prediction of molecular shape and separation of structurally related GnRHs by capillary electrophoresis Preparation and characterization of [Glu]n peptides Synthesis of peptides derived from GnRH and their analysis and characterization by capillary zone electrophoresis Capillary Electrophoresis: Affinity Electrophoresis Capillary electrophoresis (CE) and capillary electrochromatography (CEC) of amino acids, peptides and proteins Purification and analysis of antimicrobial components from flesfly Neobellieria Bullata by free-flow and capillary zone electrophoresis, and HPLC Capillary electrophoretic separation of enantiomers of nucleotide-based antiviral drugs using beta-cyclodextrin as chiral selector Determination of stability constants of valinomycin complexes with alkali metal ions by capillary electrophoresis Simul 5 - Free dynamic simulator of electrophoresis Stability constants of amino acids, peptides, proteins, and other biomolecules determined by CE and related methods: Recapitulation of published data Prediction and understanding system peaks in capillary zone electrophoresis Reliable eletrophoretic mobilities free from Joule heating effects using CE Advanced glycation end-product pentosidine accumulates in various tissues of rats with high fructose intake Comparison of CE-MS and LC-MS Analyses of Avian Eggshell Matrix Proteins Collagen : HPLC and Capillary Electromigration Methods

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