About: Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)     Goto   Sponge   NotDistinct   Permalink

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  • There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called “ageing”, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.
  • There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called “ageing”, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time. (en)
  • There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called “ageing”, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time. (cs)
Title
  • Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
  • Zlepšení vývojové kompetence stárnoucích oocytů po ošetření IGF1 a EGF (cs)
  • Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) (en)
skos:prefLabel
  • Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
  • Zlepšení vývojové kompetence stárnoucích oocytů po ošetření IGF1 a EGF (cs)
  • Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) (en)
skos:notation
  • RIV/60460709:41210/05:11703!RIV06-MSM-41210___
http://linked.open.../vavai/riv/strany
  • 300;310
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika
  • 7
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 524413
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41210/05:11703
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • epidermal growth factor, insulin-like growth factor 1, oocyte, ageing, pig (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [3A588C8604E9]
http://linked.open...i/riv/nazevZdroje
  • Czech Journal of Animal Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 50
http://linked.open...iv/tvurceVysledku
  • Jílek, František
  • Rajmon, Radko
  • Sedmíková, Markéta
  • Petrová, I.
  • Rozinek, Jiří
  • Kuthanová, Zuzana
http://linked.open...n/vavai/riv/zamer
issn
  • 1212-1819
number of pages
http://localhost/t...ganizacniJednotka
  • 41210
is http://linked.open...avai/riv/vysledek of
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