About: The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.).

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About: The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byl studován vliv chemických přídavných látek (acetosyringone,AS; L-cysteine, CYS; dithiothreitol, DTT; glutathione, GSH; cellulase, CEL; pectinase, PEC) a světelného režimu (16/8 den / noc, 16L/8D; stálé osvětlení, 24L; stálé zatemnění, 24D) během kokultivační periody explantátů hrachu s Agrobacterium tumefaciens na efektivitu transformace. Optimální podmínky byly stanoveny při 48h kokultivaci za 20+/-2°C, 50 mg l-1 CYS, 100 µM AS, 16L/8D fotoperiodě. (cs) The effect of chemical additives (acetosyringone,AS; L-cysteine, CYS; dithiothreitol, DTT;glutathione, GSH; cellulase, CEL; pectinase, PEC) and light regimes (16/8 light/dark photoperiod,16L/8D; continuous light, 24L; continuous dark, 24D) applied during cocultivation procedure of pea explants with Agrobacterium tumefaciens on transformation efficiency was studied. The improved cocultivation step for pea consists of 48 h cocultivation at 20C, with 50 mg l-1 CYS and 100 µM AS, 16L/8D photoperiod (or without AS in continuous light). The effect of chemical additives (acetosyringone,AS; L-cysteine, CYS; dithiothreitol, DTT;glutathione, GSH; cellulase, CEL; pectinase, PEC) and light regimes (16/8 light/dark photoperiod,16L/8D; continuous light, 24L; continuous dark, 24D) applied during cocultivation procedure of pea explants with Agrobacterium tumefaciens on transformation efficiency was studied. The improved cocultivation step for pea consists of 48 h cocultivation at 20C, with 50 mg l-1 CYS and 100 µM AS, 16L/8D photoperiod (or without AS in continuous light). (en)
Title	The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). Vliv kokultivačních ošetření na efektivitu transformace u hrachu (Pisum sativum L.). (cs) The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). (en)
skos:prefLabel	The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). Vliv kokultivačních ošetření na efektivitu transformace u hrachu (Pisum sativum L.). (cs) The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). (en)
skos:notation	RIV/26784246:_____/08:#0000107!RIV09-MSM-26784246
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), P(QF3072), Z(MSM2678424601)
http://linked.open...iv/cisloPeriodika	95
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Griga, Miroslav Švábová, Lenka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agritec Plant Research s.r.o.
http://linked.open...dnocenehoVysledku	365021
http://linked.open...ai/riv/idVysledku	RIV/26784246:_____/08:#0000107
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	acetosyringone, pea, genetic transformation, cocultivation (en)
http://linked.open.../riv/klicoveSlovo	cocultivation pea acetosyringone genetic transformation
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[3842F2F1C56A]
http://linked.open...i/riv/nazevZdroje	Plant Cell Tiss and Org Culture
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of transgenic pea (Pisum sativum L.) lines with enhanced resistance to viral pathogens. Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	95
http://linked.open...iv/tvurceVysledku	Griga, Miroslav Švábová, Lenka
http://linked.open...n/vavai/riv/zamer	Study and utilization of biotechnological and molecular methods in genetic and breeding research of genus Pisum and Linum
issn	0167-6857
number of pages	12 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.). The effect of cocultivation treatments on transformation efficiency in pea (Pisum sativum L.).

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