About: Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments

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About: Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach. Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach. (en)
Title	Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments (en)
skos:prefLabel	Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments (en)
skos:notation	RIV/00216275:25310/14:39897974!RIV15-MSM-25310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE2.3.30.0021)
http://linked.open...iv/cisloPeriodika	November
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Rotková, Jana Podzimek, Štěpán Korecká, Lucie Bílková, Zuzana Holubová, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	14673
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/14:39897974
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Pharmaceutics; SEC-MALS; Magnetic particles; Papain; Hyaluronan fragmentation (en)
http://linked.open.../riv/klicoveSlovo	Hyaluronan fragmentation Papain SEC-MALS Magnetic particles Pharmaceutics
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[7AD0AE6496F3]
http://linked.open...i/riv/nazevZdroje	Carbohydrate Polymers
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Strenthening of Research and Development Teams at the University of Pardubice
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	112
http://linked.open...iv/tvurceVysledku	Podzimek, Štěpán Velebný, Vladimír Bílková, Zuzana Ehlová, Tereza Korecká, Lucie Holubová, Lucie Rotková, Jana Moravcová, Veronika
http://linked.open...ain/vavai/riv/wos	000341464600037
issn	0144-8617
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.carbpol.2014.05.096
http://localhost/t...ganizacniJednotka	25310

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