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Description
  • určeno pro seznam publikací (cs)
  • Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphologic
  • Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphologic (en)
Title
  • Occurence of Fusarium in food and feed
  • Occurence of Fusarium in food and feed (en)
  • Plísně rodu Fusarium v potravinách a krmivech (cs)
skos:prefLabel
  • Occurence of Fusarium in food and feed
  • Occurence of Fusarium in food and feed (en)
  • Plísně rodu Fusarium v potravinách a krmivech (cs)
skos:notation
  • RIV/00216275:25310/05:00002650!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany
  • 37-38
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/05/2106), Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 533927
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/05:00002650
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Fusarium spp.; Fusarium graminearum; toxinogenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [46B173C38013]
http://linked.open...v/mistoKonaniAkce
  • Olomouc
http://linked.open...i/riv/mistoVydani
  • Praha
http://linked.open...i/riv/nazevZdroje
  • Czech Mycology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Vytřasová, Jarmila
  • Červenka, Libor
  • Svobodová, Lucie
  • Brožková, Iveta
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
issn
  • 1211-0981
number of pages
http://purl.org/ne...btex#hasPublisher
  • Česká vědecká společnost pro mykologii
http://localhost/t...ganizacniJednotka
  • 25310
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