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  • Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e. g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Forster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying.
  • Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e. g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Forster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying. (en)
Title
  • Multi-step synthesis of Caspase-3 sensor based on Forster resonance energy transfer
  • Multi-step synthesis of Caspase-3 sensor based on Forster resonance energy transfer (en)
skos:prefLabel
  • Multi-step synthesis of Caspase-3 sensor based on Forster resonance energy transfer
  • Multi-step synthesis of Caspase-3 sensor based on Forster resonance energy transfer (en)
skos:notation
  • RIV/00216224:14310/13:00072526!RIV14-MSM-14310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 90188
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/13:00072526
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Apoptosis; Caspases; Chemiluminiscence; Forster Resonance Energy Transfer; Multistep synthesis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [C8BE7FDA8ACF]
http://linked.open...i/riv/nazevZdroje
  • Chemické listy
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 107
http://linked.open...iv/tvurceVysledku
  • Foret, František
  • Klepárník, Karel
  • Lišková, Marcela
  • Pazdera, Pavel
http://linked.open...ain/vavai/riv/wos
  • 000328730800013
issn
  • 0009-2770
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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