About: aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://jb.asm.org/cgi/content/abstract/190/9/3155
Description	In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response. In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response. (en)
Title	aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity (en)
skos:prefLabel	aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity (en)
skos:notation	RIV/00216224:14310/08:00050960!RIV12-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06030), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Budinská, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	357020
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00050960
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	aro mutant; Salmonella enterica; microarray; gene expression; plasmid (en)
http://linked.open.../riv/klicoveSlovo	Salmonella enterica gene expression plasmid microarray aro mutant
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D4E455FE3FF6]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF BACTERIOLOGY
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomolecular centre
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	190
http://linked.open...iv/tvurceVysledku	Crhánová, Magdaléna Karasová, Daniela Rychlík, Ivan Budinská, Eva Šebková, Alena
http://linked.open...ain/vavai/riv/wos	000255202100008
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0021-9193
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1128/JB.00053-08
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

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