About: Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding

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About: Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The integrity of sperm acrosome is required for normal sperm-egg interaction. Antibodies to acrosomal proteins are still frequently utilized to check the acrosomal state. We studied acrosomal proteins in boar and human spermatozoa by using of Hs-8 and Hs-14 monoclonal antibodies (MoAbs) which were prepared in our laboratory.The intra-acrosomal proteins recognized by Hs-8 and Hs-14 MoAbs are stable upon capacitation and even after induction of acrosome reaction. The role of pHs-8 and pHs-14 proteins in sperm-egg binding was investigated by in vitro binding assay of boar spermatozoa and porcine oocytes. The Hs-14 relevant protein does not participate in gamete binding while the Hs-8 detected protein is a secondary binding protein The integrity of sperm acrosome is required for normal sperm-egg interaction. Antibodies to acrosomal proteins are still frequently utilized to check the acrosomal state. We studied acrosomal proteins in boar and human spermatozoa by using of Hs-8 and Hs-14 monoclonal antibodies (MoAbs) which were prepared in our laboratory.The intra-acrosomal proteins recognized by Hs-8 and Hs-14 MoAbs are stable upon capacitation and even after induction of acrosome reaction. The role of pHs-8 and pHs-14 proteins in sperm-egg binding was investigated by in vitro binding assay of boar spermatozoa and porcine oocytes. The Hs-14 relevant protein does not participate in gamete binding while the Hs-8 detected protein is a secondary binding protein (en) Celistvost akrozomu spermie je nezbytná pro normální interakci spermie s vajíčkem. K ověření stavu akrozomu se ještě stále často používají protilátky proti proteinům akrozomu. Studovali jsme akrozomální proteiny pomocí protilátek Hs-8 a Hs-14 připravených v naší laboratoři. Proteiny uvnitř akrozomu rozpoznané Hs-8 a Hs-14 jsou při kapacitaci a dokonce i po indukci akrozomové reakce stabilní. Byla zkoumána úloha proteinů pHs-8 a pHs-14 ve vazbě spermie na vajíčko pomocí vazebného testu kančích spermií a prasečích oocytů in vitro. Protein zjištěny Hs-14 se vazby gamet neúčastní, zatímco protein detekovaný Hs-8 je proteinem sekundární vazby (cs)
Title	Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding Celistvost akrozomu savčích spermií hodnocená monoklonálními protilátkami proti vnitřním proteinům akrozomu a úloha těchto proteinů ve vazbě spermie a vajíčka (cs) Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding (en)
skos:prefLabel	Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding Celistvost akrozomu savčích spermií hodnocená monoklonálními protilátkami proti vnitřním proteinům akrozomu a úloha těchto proteinů ve vazbě spermie a vajíčka (cs) Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding (en)
skos:notation	RIV/68378050:_____/04:00109714!RIV/2005/GA0/A23005/N
http://linked.open.../vavai/riv/strany	480;481
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/02/1373), P(NJ7463)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Pěknicová, Jana Geussová, Gizela Čapková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	553431
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/04:00109714
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	monoclonal antibody; human spermatozoa; sperm protein (en)
http://linked.open.../riv/klicoveSlovo	sperm protein monoclonal antibody human spermatozoa
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1DC8E1E9E6A3]
http://linked.open...i/riv/nazevZdroje	American Journal of Reproductive Immunology
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Male infertility, sperm and seminal plasma status, damage biomarkers. Sperm proteins and ubiquitination of acromose in human sperm with normal and pathological spermiogram
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Pěknicová, Jana Čapková, Jana Geussová, Gizela
issn	1046-7408
number of pages	2 (xsd:int)

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