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| rdf:type
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| Description
| - Native and denatured states of bovine serum albumin (BSA) were studied by d.c. polarographic and voltammetric Brdicka catalytic responses (BCR) in cobalt-containing solution and by constant current chronopotentiometric stripping analysis (CPSA) in borate buffer, pH 9.3. We found that 90 nM denatured BSA produced catalytic peak H (around -1.8 V vs. Ag/AgCl/3M KCl). This peak was about 50-fold higher than the native protein under the same conditions. Qualitatively similar results were obtained also with other proteins in native and denatured states, such as human serum albumin, gamma-globulin, myoglobin and alpha-crystallin. 2 nM denatured BSA produced a well-developed CPS peak (at accumulation time 5 min) while native BSA yielded almost no signal under the same conditions.
- Native and denatured states of bovine serum albumin (BSA) were studied by d.c. polarographic and voltammetric Brdicka catalytic responses (BCR) in cobalt-containing solution and by constant current chronopotentiometric stripping analysis (CPSA) in borate buffer, pH 9.3. We found that 90 nM denatured BSA produced catalytic peak H (around -1.8 V vs. Ag/AgCl/3M KCl). This peak was about 50-fold higher than the native protein under the same conditions. Qualitatively similar results were obtained also with other proteins in native and denatured states, such as human serum albumin, gamma-globulin, myoglobin and alpha-crystallin. 2 nM denatured BSA produced a well-developed CPS peak (at accumulation time 5 min) while native BSA yielded almost no signal under the same conditions. (en)
- Nativní a denaturovaný hovězí sérový albumin (BSA) byly studovány polarografickou a voltametrickou Brdičkovou katalytickou reakcí v roztocích obsahujících kobalt a chronopotenciometrickou stripovací analýzou (CPSA) konstantním proudem v borátovém pufru pH 9,3. Zjistili jsme, že 90 nM denaturovaného BSA poskytlo katalytický pík H (kolem -1,8 V proti Ag/AgCl/š M KCl). Tento pík byl asi 50x vyšší než nativního proteinu za týchž podmínek. Kvalitativně podobné výsledky byly získány také s dalšími bílkovinami v nativním a denaturovaném stavu, a to jmenovitě lidský serumalbumin, gamma-globulin, myoglobin a alfa-krystalin. 2 nM denaturovaný BSA vykazoval dobře vyvinutý CPS pík (při době akumulace 5 min), zatímco nativní BSA za stejných podmínek neposkytl téměř žádný signál. (cs)
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| Title
| - Native and denatured bovine serum albumin. D.c. polarography, stripping voltammetry and constant current chronopotentiometry
- Native and denatured bovine serum albumin. D.c. polarography, stripping voltammetry and constant current chronopotentiometry (en)
- Nativní a denaturovaný hovězí serumalbumin. D.c. polarografie, striping voltametrie a chronopotenciometrie konstantním proudem (cs)
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| skos:prefLabel
| - Native and denatured bovine serum albumin. D.c. polarography, stripping voltammetry and constant current chronopotentiometry
- Native and denatured bovine serum albumin. D.c. polarography, stripping voltammetry and constant current chronopotentiometry (en)
- Nativní a denaturovaný hovězí serumalbumin. D.c. polarografie, striping voltametrie a chronopotenciometrie konstantním proudem (cs)
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| skos:notation
| - RIV/68081707:_____/06:00047938!RIV07-AV0-68081707
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(IAA500040513), P(LC06035), Z(AV0Z50040507)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68081707:_____/06:00047938
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - protein electrochemistry; bovine serum albumin; native and denatured proteins (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - CH - Švýcarská konfederace
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Journal of the Electroanalytical Chemistry and Journal of Electroanalytical Chemistry and Interfacial Electrochemistry
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Ostatná, Veronika
- Paleček, Emil
- Dogan, B.
- Ozkan, S.
- Uslu, B.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| is http://linked.open...avai/riv/vysledek
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