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Description
| - Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract–(NH4)2SO4–glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium
- Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract–(NH4)2SO4–glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium (en)
- Streptomyces caelestis DSM 40 084 produkuje dva osmolyty: 2-O-(D-glukopyranosyl-glycerolovou kyselinu (GG) a trehalosu. Obě sloučeniny byly izolovány a identifikovány nukleární magnetickou resonancí a hmotnostní spektrometrií. Byla zjištěna velmi citlivá regulace buněčných osmolytů v exponenciálně rostoucích kulturách. Intracelulární obsah GG a trehalosy se zvýšil dvakrát po skokové změně osmolarity média vyvolané 0.3 procent NaCl. 1H NMR analýza buněčných extraktů nepotvrdila přítomnost dalších osmolytů. GG je metabolit uvolňovaný z buněk a jeho koncentrace dosahuje 3g/L při kultivaci v médiu obsahující yeast extrakt, (NH4)2SO4 a glycerol. Tato práce poprvé informuje o výskytu iontového osmolytu GG u rodu Streptomyces a o jeho volné exkreci do média (cs)
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Title
| - Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis
- Glukosylglycerát je osmolit a extracelulární metabolit produkovaný Streptomyces caelestis (cs)
- Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis (en)
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skos:prefLabel
| - Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis
- Glukosylglycerát je osmolit a extracelulární metabolit produkovaný Streptomyces caelestis (cs)
- Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis (en)
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skos:notation
| - RIV/61388971:_____/07:00096764!RIV08-AV0-61388971
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(IAA600660607), Z(AV0Z50200510)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/07:00096764
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - streptomyces caelestis; mass spectrometry (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Halada, Petr
- Pospíšil, Stanislav
- Petříček, Miroslav
- Sedmera, Petr
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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