About: Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic poly(2-hydroxyethyl methacrylate-) and poly(glycidyl methacrylate-) based microparticles were prepared by dispersion polymerization in the presence of iron oxide nanoparticles, both commercial and laboratory-made. The polymerization was highly sensitive to even subtle changes in the various reaction parameters involved in the process. Several DNA applications of developed microparticles were described. The sensitivity of polymerase chain reaction (PCR) in cell detection was negatively affected by some magnetic carriers and compounds used in their preparation. Carboxyl group-containing magnetic microparticles were prepared for isolation of genomic DNA from cell lysates. Magnetic poly(2-hydroxyethyl methacrylate-) and poly(glycidyl methacrylate-) based microparticles were prepared by dispersion polymerization in the presence of iron oxide nanoparticles, both commercial and laboratory-made. The polymerization was highly sensitive to even subtle changes in the various reaction parameters involved in the process. Several DNA applications of developed microparticles were described. The sensitivity of polymerase chain reaction (PCR) in cell detection was negatively affected by some magnetic carriers and compounds used in their preparation. Carboxyl group-containing magnetic microparticles were prepared for isolation of genomic DNA from cell lysates. (en)
Title	Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics (en)
skos:prefLabel	Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics (en)
skos:notation	RIV/00216224:14310/05:00012408!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/02/0287), P(LN00A065)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	528612
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00012408
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Magnetic carriers; DNA; microparticles (en)
http://linked.open.../riv/klicoveSlovo	Magnetic carriers microparticles DNA
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[34BB2E92335B]
http://linked.open...i/riv/nazevZdroje	Polymer
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Cell Therapy and Tissue Repair Development of screening ELISA and PCR methods for detection of Campylobacteria in foods
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Španová, Alena Beneš, Milan
issn	0032-3861
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics

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